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Invitrogen
Ubiquitin Monoclonal Antibody (5B9-B3), PerCP
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FIGURE: 1 / 3
Ubiquitin Antibody (MA5-45204) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with Ubiquitin monoclonal antibody (Product # MA5-45204) at 1:100 for 12 hours at 4°C, followed by FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Dif... View More
Product Details
MA5-45204
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
All,
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
5B9-B3
Immunogen
Native bovine ubiquitin, conjugated to KLH
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.2, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45204 was sufficient for detection of ubiquitin in 10 µg of Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 10kDa.
Target Information
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 40S ribosomal protein S27-like; 40S ribosomal protein S27-like protein; 40S ribosomal protein S27a; 60S ribosomal protein L40; CEP52; Chap1; epididymis luminal protein 112; epididymis secretory protein Li 50; hPLIC-2; HRIHFB2157; HUBCEP52; OTTHUMP00000200786; OTTHUMP00000201692; PLIC-2; polyubiquitin; polyubiquitin B; polyubiquitin C; Polyubiquitin-B; Polyubiquitin-C; pro-ubiquitin; Ribosomal protein eS27-like; ribosomal protein S27; RPL40; RPS27A; rps27l; Small ribosomal subunit protein eS27-like; UBA52; UBB; UBC; Ubiquilin 2; ubiquitin; Ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein L40; ubiquitin and ribosomal protein S27a; ubiquitin C; ubiquitin carboxyl extension protein 52; Ubiquitin carboxyl extension protein 80; ubiquitin precursor; ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a-like; ubiquitin-52 amino acid fusion protein; ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-CEP80; Ubiquitin-ribosomal protein eL40 fusion protein; Ubiquitin-ribosomal protein eS31 fusion protein; ubiquitin/60S ribosomal fusion protein; unnamed protein product
Gene Aliases: 0610006J14Rik; 1810034D23Rik; 2700054O04Rik; AI194771; AL033289; CEP52; CEP80; D8Ertd21e; eS31; Gm1863; HEL-S-50; HEL112; HMG20; HUBCEP52; L40; RPL40; RPS27A; RPS27L; S27A; TI-225; UBA52; UBA80; UBB; Ubb2; UBC; UBCEP1; UBCEP2; UBCEP80
UniProt ID: (Rat) P62982, (Bovine) P62992, (Human) P62979, (Bovine) Q3T0B7, (Human) Q71UM5, (Mouse) P62983, (Rat) P24051, (Mouse) Q6ZWY3, (Human) P62987, (Bovine) P63048, (Bovine) P0CG53, (Human) P0CG47, (Human) P0CG48, (Bovine) P0CH28, (Rat) P62986, (Mouse) P62984, (Rat) P0CG51, (Mouse) P0CG49, (Mouse) P0CG50, (Rat) Q63429
Entrez Gene ID: (Rat) 100912032, (Bovine) 286839, (Human) 6233, (Bovine) 614220, (Human) 51065, (Mouse) 78294, (Rat) 681429, (Mouse) 67941, (Human) 7311, (Bovine) 615199, (Bovine) 281370, (Human) 7314, (Human) 7316, (Bovine) 444874, (Rat) 64156, (Mouse) 22186, (Rat) 192255, (Mouse) 22187, (Mouse) 22190, (Rat) 50522
structural constituent of ribosome
metal ion binding
RNA binding
protein binding
zinc ion binding
protein tag
ubiquitin protein ligase binding
translation activator activity
cysteine-type endopeptidase activator activity involved in apoptotic process
poly(A) RNA binding
protease binding
ribosomal protein
translational protein
translation
cytoplasmic translation
protein ubiquitination
modification-dependent protein catabolic process
ribosomal small subunit biogenesis
activation of cysteine-type endopeptidase activity involved in apoptotic process
DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
mitotic G1 DNA damage checkpoint
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of translation
ribosomal small subunit assembly
DNA damage response, signal transduction by p53 class mediator
biological_process
DNA repair
cellular response to DNA damage stimulus
response to insecticide
protein modification process
male meiosis I
female meiosis I
female gonad development
hypothalamus gonadotrophin-releasing hormone neuron development
mitochondrion transport along microtubule
neuron projection morphogenesis
regulation of mitochondrial membrane potential
fat pad development
regulation of proteasomal protein catabolic process
seminiferous tubule development
energy homeostasis
regulation of neuron death
positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator
positive regulation of protein monoubiquitination
male gonad development
positive regulation of protein ubiquitination
regulation of neuron apoptotic process
adipose tissue development
ubiquitin homeostasis
ubiquitin-dependent protein catabolic process
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