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Invitrogen
CRYAB Monoclonal Antibody (3A10.C9), APC
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FIGURE: 1 / 2
CRYAB Antibody (MA5-45217) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Alpha B Crystallin monoclonal antibody (Product # MA5-45217) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha B ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45217
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3A10.C9
Immunogen
Native Alpha B Crystallin
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.2, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
0.5 µg/mL of MA5-45217 was sufficient for detection of 50 ng purified alpha B crystalline by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 20kDa (predicted mol.weight is approximately 21kDa). Does not cross-react with alpha A-crystallin, beta L-crystallin, beta H-crystallin, gamma -crystallin, HSP25, HSP27 or HSP47 proteins.
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha crystallin B; Alpha(B)-crystallin; Alpha-crystallin B chain; CryAB antibody; crystallin, alpha polypeptide 2; Heat shock protein beta-5; Heat shock protein family B member 5; HspB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component
Gene Aliases: AACRYA; CRYA2; CRYAB; HSPB5
UniProt ID: (Bovine) P02510, (Rat) P23928
Entrez Gene ID: (Bovine) 281719, (Rat) 25420
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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