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Invitrogen
GRP78 Monoclonal Antibody (6H4-2G7), APC
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FIGURE: 1 / 2
GRP78 Antibody (MA5-45280) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with GRP78 monoclonal antibody (Product # MA5-45280) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was DAPI (blue) nuclear stain at 1:5,000 for 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45280
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Fungi,
Hamster,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat,
Xenopus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
6H4-2G7
Immunogen
His-tagged human GRP78
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
0.5 µg/mL of MA5-45280 was sufficient for detection of Grp78 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.|Detects approximately 78kDa.
Target Information
GRP78 is a 78 kDa glucose regulated protein that belongs to the family of heat shock proteins that include HSP70. GRP78 is a resident protein of the endoplasmic reticulum (ER) and associates transiently with a variety of newly synthesized secretory and membrane proteins. GRP78 may also interact with mutant and misfolded proteins, marking them for degradation. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP94) and protein disulfide isomerase (PDI). The KDEL signal is recognized by the KDEL receptor and this interaction ensures recycling of escaped ER proteins back to the ER. GRP78's involvement in correct folding of proteins and degradation of misfolded proteins is via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. GRP78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. GRP78 protein levels have been shown to be a reliable biomarker of hypoglycemia and serves a neuroprotective function in neurons exposed to glutamate and oxidative stress. GRP78 levels are reduced in the brains of Alzheimer's Disease patients, and decreased expression of GRP78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. Further, the induction of the GRP78 protein has been associated with the development of drug-resistance to antitumor drugs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 78 kDa glucose-regulated protein; Binding-immunoglobulin protein; BiP; BiP protein; Endoplasmic reticulum chaperone BiP; FLJ26106; glucose regulated protein, 78 kDa; glucose-regulated protein; GRP 78; GRP-78; heat shock 70 kDa protein 5; heat shock 70 kDa protein 5a; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); heat shock protein 5; Heat shock protein 70 family protein 5; Heat shock protein family A member 5; heavy-chain binding protein BiP; HSP70 family protein 5; HSPA 5; immunoglobulin binding protein; Immunoglobulin heavy chain-binding protein; Steroidogenesis-activator polypeptide; XAP-1 antigen
Gene Aliases: AL022860; AU019543; baffled; BIP; BiP/Grp78; D2Wsu141e; D2Wsu17e; GRP78; Hsce70; HSPA5; hspa5a; I79_019946; mBiP; SEZ-7; Sez7; XAP-1; XELAEV_180384511mg
UniProt ID: (Bovine) Q0VCX2, (Rat) P06761, (Mouse) P20029, (Chinese hamster) G3I8R9, (Xenopus) Q91883
Entrez Gene ID: (Rabbit) 100008764, (Bovine) 415113, (Rat) 25617, (Mouse) 14828, (Chinese hamster) 100689305, (Xenopus) 397850
ER overload response
activation of signaling protein activity involved in unfolded protein response
cerebellum structural organization
cerebellar Purkinje cell layer development
substantia nigra development
positive regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
positive regulation of protein ubiquitination
response to endoplasmic reticulum stress
maintenance of protein localization in endoplasmic reticulum
cellular response to glucose starvation
negative regulation of apoptotic process
proteolysis involved in cellular protein catabolic process
cellular response to antibiotic
cellular response to interleukin-4
toxin transport
positive regulation of embryonic development
posttranslational protein targeting to membrane, translocation
negative regulation of protein complex assembly
negative regulation of IRE1-mediated unfolded protein response
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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