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Invitrogen
HSP70 Monoclonal Antibody (1H11), FITC
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45368
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1H11
Immunogen
Human native HSP70 protein
Conjugate
FITC
FITC
FITC
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
9.09mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:250 dilution of MA5-45368 was sufficient for detection of cell surface HSP70 in HCT116 cells using Fluorescence-activated cell sorting, applied in flow cytometry, with FITC as the fluorescent probe.|Conformation-specific antibody for membrane-bound HSP70. Binds to cell surface HSP70 without permeabilization of cell membrane in tumor cell lines. Does not bind to intracellular HSP70 at low concentrations. Cross-reacts with HSC70. Detects a approximately 70 kDa band in Western Blot.
Target Information
HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 68 kDa heat shock protein; 70 kda heat shock protein-2; 70 kilodalton heat shock protein; ARABIDOPSIS HEAT SHOCK PROTEIN 70; ATHSP70; chaperone; heat shock 10kDa protein 1-like; heat shock 70 kD protein 1; heat shock 70 kD protein 2; heat shock 70 kD protein 3; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; Heat shock 70 kDa protein 4; Heat shock 70 kDa-like protein 1; Heat shock 70-related protein APG-2; heat shock 70kD protein 1B; heat shock 70kDa protein 1-like; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 2; heat shock 70kDa protein 3; heat shock 70kDa protein 4; Heat shock protein; Heat Shock Protein 70; heat shock protein 70-1; Heat shock protein 70-1l; heat shock protein 70.1; Heat shock protein 70.2; heat shock protein alpha 2; heat shock protein cognate 70, testis; Heat shock protein family A member 1A; Heat shock protein family A member 1B; Heat shock protein family A member 1L; Heat shock protein family A member 2; Heat shock protein family H member 2; heat shock protein, 110 kDa; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 2; heat shock protein, 70 kDa 3; Heat shock-related 70 kDa protein 2; heat shock-related protein hsc70t; heat-shock 70-kilodalton protein 1A; heat-shock 70-kilodalton protein 1B; HEL S 103; HSP; Hsp68; HSP70 1; HSP70 1B; HSP70 2; HSP70 AceyltK77; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70-Hom; HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; HSP70.2; HSP70.3; HSP70RY; HSPA2; HSPA4; Hspa70; HST; inducible heat shock protein 70; Ischemia responsive 94 kDa protein; Spermatid-specific heat shock protein 70; Testis-specific heat shock protein-related; testis-specific heat shock protein-related gene hst70
Gene Aliases: 70kDa; AI317151; APG-2; APG2; Hcp70.1; Hcp70.2; Hsc70t; Hsp110; hsp68; HSP70; HSP70-1; HSP70-2; HSP70-3; Hsp70.1; HSP70.2; Hsp70.3; Hsp70A1; HSP70A2; Hsp70RY; HSP72; HSPA1; HSPA1A; HSPA1B; HSPA1L; HSPA2; HSPA3; HSPA4; HSPH2; Hspt70; Hst70; HSX70; Irp94; mKIAA4025; Msh5
ATP binding
G-protein coupled receptor binding
protease binding
double-stranded RNA binding
ATPase activity
enzyme binding
heat shock protein binding
ubiquitin protein ligase binding
ATPase activity, coupled
protein binding involved in protein folding
poly(A) RNA binding
protein N-terminus binding
NF-kappaB binding
unfolded protein binding
C3HC4-type RING finger domain binding
protein binding
molecular_function
nucleotide binding
glycolipid binding
RNA polymerase II transcription factor binding
protein complex binding
activating transcription factor binding
binding of sperm to zona pellucida
biological_process
protein refolding
spermatogenesis
response to heat
response to cold
cell differentiation
telomere maintenance
DNA repair
protein folding
negative regulation of protein phosphorylation
positive regulation of gene expression
negative regulation of gene expression
positive regulation of protein binding
negative regulation of apoptotic process
negative regulation of DNA binding
protein import into mitochondrial outer membrane
chaperone-mediated protein complex assembly
negative regulation of cell death
response to ischemia
mRNA catabolic process
response to unfolded protein
negative regulation of cell proliferation
response to radiation
response to mechanical stimulus
regulation of cell death
neuron differentiation
negative regulation of cell growth
negative regulation of protein ubiquitination
cellular response to heat
response to ethanol
positive regulation of erythrocyte differentiation
positive regulation of neuron differentiation
cellular heat acclimation
negative regulation of inclusion body assembly
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of protein targeting to mitochondrion
kidney development
positive regulation of angiogenesis
positive regulation of microtubule nucleation
regulation of mitotic spindle assembly
positive regulation of protein phosphorylation
male meiosis
male meiosis I
multicellular organism development
spermatid development
positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle
synaptonemal complex disassembly
positive regulation of T cell mediated cytotoxicity
defense response
negative regulation of myeloid cell apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of vasoconstriction
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