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Invitrogen
CD74 Monoclonal Antibody (3D7), PerCP
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FIGURE: 1 / 2
CD74 Antibody (MA5-45383) in Flow
Flow Cytometry analysis using human neuroblastoma cells. Fixation involved 90% Methanol. Samples were incubated with CD74 monoclonal antibody (Product # MA5-45383) at 1:100 for 30 min on ice, followed by Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to heat shock at 42°C for 2 hours and transferred to CO2 incubator at 37°C for 1 hour before trypsinization.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45383
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3D7
Immunogen
Human CD74 invariant chain synthetic peptide.
Conjugate
PerCP
PerCP
PerCP
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:1,000 dilution of MA5-45383 was sufficient for detection of CD74 in 15 µg of B-Cell Lymphoma cell line Raji by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Detects approximately 33 kDa protein.
Target Information
HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; class II MHC-associated invariant chain peptide; gamma chain of class II antigens; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia-associated invariant chain; MHC HLA-DR gamma chain; unnamed protein product
Gene Aliases: CLIP; DHLAG; HLADG; Ia-GAMMA; II; p33
UniProt ID: (Human) P04233
Entrez Gene ID: (Human) 972
beta-amyloid binding
cytokine receptor activity
protein binding
cytokine binding
MHC class II protein complex binding
macrophage migration inhibitory factor binding
MHC class II protein binding
CD4 receptor binding
MHC class II protein binding, via antigen binding groove
identical protein binding
protein binding involved in protein folding
nitric-oxide synthase binding
prostaglandin biosynthetic process
positive regulation of cytokine-mediated signaling pathway
T cell activation involved in immune response
positive regulation of dendritic cell antigen processing and presentation
negative regulation of peptide secretion
positive regulation of type 2 immune response
negative regulation of mature B cell apoptotic process
protein folding
intracellular protein transport
immune response
positive regulation of gene expression
immunoglobulin mediated immune response
antigen processing and presentation
antigen processing and presentation of endogenous antigen
negative regulation of cell migration
positive regulation of B cell proliferation
positive regulation of prostaglandin biosynthetic process
positive regulation of chemokine production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
response to interferon-gamma
macrophage migration inhibitory factor signaling pathway
regulation of macrophage activation
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
negative regulation of DNA damage response, signal transduction by p53 class mediator
T cell selection
positive regulation of monocyte differentiation
negative regulation of viral entry into host cell
positive regulation of fibroblast proliferation
protein stabilization
positive regulation of macrophage cytokine production
protein-containing complex assembly
protein trimerization
positive regulation of ERK1 and ERK2 cascade
positive regulation of neutrophil chemotaxis
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of chemokine (C-X-C motif) ligand 2 production
positive regulation of macrophage migration inhibitory factor signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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