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Invitrogen
HCN1 Monoclonal Antibody (S70), PE
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FIGURE: 1 / 6
HCN1 Antibody (MA5-45402) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with HCN1 monoclonal antibody (Product # MA5-45402) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN1 antibody (D) Composite.
Product Details
MA5-45402
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
S70
Immunogen
Fusion protein amino acids 778-910 (C terminus) of rat HCN1
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45402 was sufficient for detection of HCN1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 100kDa. No cross-reactivity against HCN2.
Target Information
HCN1 includes Hyperpolarization-activated cation channels of the HCN gene family, which contribute to spontaneous rhythmic activity in both heart and brain. HCN1 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations. The HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BCNG-1; Brain cyclic nucleotide-gated channel 1; HAC-2; Hyperpolarization-activated cation channel 2; potassium channel; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Gene Aliases: BCNG-1; BCNG1; C630013B14Rik; DEE24; EIEE24; GEFSP10; HAC-2; HAC2; HCN1
UniProt ID: (Human) O60741, (Mouse) O88704, (Rat) Q9JKB0
Entrez Gene ID: (Human) 348980, (Mouse) 15165, (Rat) 84390
nucleotide binding
ion channel activity
intracellular cAMP activated cation channel activity
voltage-gated sodium channel activity
voltage-gated potassium channel activity
potassium channel activity
sodium channel activity
protein binding
phosphatidylinositol-4,5-bisphosphate binding
phosphatidylinositol-3,4,5-trisphosphate binding
voltage-gated cation channel activity
cAMP binding
identical protein binding
intracellular cAMP-activated cation channel activity involved in regulation of presynaptic membrane potential
voltage-gated ion channel activity
protein C-terminus binding
protein complex binding
ion channel
transporter
regulation of membrane depolarization
ion transport
potassium ion transport
sodium ion transport
neuronal action potential
ion transmembrane transport
cellular response to interferon-beta
sodium ion transmembrane transport
regulation of membrane potential
maternal behavior
apical protein localization
negative regulation of action potential
retinal cone cell development
protein homotetramerization
response to calcium ion
general adaptation syndrome, behavioral process
negative regulation of synaptic transmission, glutamatergic
transmembrane transport
regulation of postsynaptic membrane potential
cellular response to cAMP
potassium ion transmembrane transport
regulation of heart rate by cardiac conduction
sodium ion import across plasma membrane
regulation of SA node cell action potential
response to L-glutamate
regulation of membrane hyperpolarization
positive regulation of membrane hyperpolarization
potassium ion import across plasma membrane
transport
regulation of ion transmembrane transport
positive regulation of cation channel activity
inflammatory response
aging
response to cisplatin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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