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Invitrogen
Kir2.1 (KCNJ2) Monoclonal Antibody (S112), APC
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FIGURE: 1 / 4
Kir2.1 (KCNJ2) Antibody (MA5-45415) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with Kir2.1 monoclonal antibody (Product # MA5-45415) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM f... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45415
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
S112
Immunogen
Fusion protein amino acids 41-64 and 189-428 of mouse Kir2.1
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45415 was sufficient for detection of Kir2.1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 45kDa. No cross-reactivity against Kir2.2 or Kir2.3.
Target Information
The KIR2 subunit family includes 2. 1, 2. 2, 2. 3 and 2. 4. Unlike G protein-coupled KIR3 subunits, KIR2. 1 requires both phosphorylation by PKA and ATP hydrolysis for functional activity. KIR2. 1 is expressed in the superior and inferior collicula and the pontine region of the brain, where it moderates synaptic transmission, like many other potassium channels. In the placenta, KIR2. 1 is expressed throughout gestation in cytotrophoblast cells. In the kidney, KIR2. 1 colocalizes with KIR5. 1 in the proximal tubule. KIR2. 1, 2. 2 and 2. 3 associate with the membrane-associated guanylate kinase synapseassociated protein 97 in the cerebellum and heart. Phosphorylation of KIR2. 2 by protein kinase A inhibits the associates with SAP97.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cardiac inward rectifier potassium channel; HEos-IRK; HHIRK 1; HIRK 1; hIRK1; Inward rectifier K; inward rectifier K(+) channel Kir2.1; inward rectifier K+ channel KIR2.1; IRK 1; IRK-1; IRK2; LQT 7; potassium channel, inwardly rectifying subfamily J member 2; potassium channel, inwardly rectifying subfamily J, member 2; potassium voltage-gated channel subfamily J member 2; RBL-IRK1; SQT 3; unnamed protein product
Gene Aliases: ATFB9; HHBIRK1; HHIRK1; IRK1; Kcnf1; KIR2.1; LQT7; SQT3
UniProt ID: (Human) P63252, (Mouse) P35561, (Rat) Q64273
Entrez Gene ID: (Human) 3759, (Mouse) 16518, (Rat) 29712
potassium ion transport
regulation of skeletal muscle contraction via regulation of action potential
magnesium ion transport
protein homotetramerization
relaxation of cardiac muscle
regulation of membrane repolarization
cellular response to mechanical stimulus
potassium ion transmembrane transport
cardiac muscle cell action potential
cardiac muscle cell action potential involved in contraction
regulation of cardiac muscle cell contraction
membrane repolarization during action potential
membrane repolarization during cardiac muscle cell action potential
regulation of heart rate by cardiac conduction
relaxation of skeletal muscle
positive regulation of potassium ion transmembrane transport
potassium ion import across plasma membrane
cellular potassium ion homeostasis
regulation of resting membrane potential
membrane depolarization during cardiac muscle cell action potential
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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