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Invitrogen
SCN9A Monoclonal Antibody (N68/6), PE
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FIGURE: 1 / 5
SCN9A Antibody (MA5-45422) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with Nav1.7 monoclonal antibody (Product # MA5-45422) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Nav1.7 antibody (D) Composite.
Product Details
MA5-45422
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N68/6
Immunogen
Fusion protein amino acids 1751-1946 (C-terminus) of human Nav1.7
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45422 was sufficient for detection of Nav1.7 in 10 µg of HEK-293 cell lysate transiently expressing Nav1.7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 230kDa. No cross-reactivity against other Nav channels.
Target Information
Epithelial sodium channels are amiloride-sensitive members of the Degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. Members of this superfamily of ion channels share organizational similarity in that they all possess two short intracellular amino and carboxyl termini, two short membrane spanning segments, and a large extracellular loop with a conserved cysteine-rich region. There are three homologous isoforms of the ENaC (alpha, beta, and gamma) protein. ENaC in the kidney, lung, and colon plays an essential role in trans-epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is thought to be regulated, in part, through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddels syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hNaN; hNE Na; hNE-Na; KIAA1356; MED; NAC1; NAC2; NAC3; Nav1.1; Nav1.2; Nav1.3; Nav1.4; Nav1.5; Nav1.6; Nav1.8; Nav1.9; NE; NE NA; Neuroendocrine sodium channel; Peripheral sodium channel 1; PN1; SCN1; SCN10A; SCN11A; SCN12A; SCN1A; SCN2A1; SCN2A2; SCN3A; SCN4A; SCN5A; SCN8A; SNS2; sodium channel 25; Sodium channel protein type 9 subunit alpha; Sodium channel protein type IX subunit alpha; sodium channel voltage-gated type IX alpha; sodium channel, voltage-gated, type 9, alpha polypeptide; sodium channel, voltage-gated, type IX, alpha polypeptide; sodium channel, voltage-gated, type IX, alpha subunit; voltage-gated sodium channel alpha subunit Nav1.7; Voltage-gated sodium channel subunit alpha Nav1.7
Gene Aliases: ETHA; FEB3B; GEFSP7; HSAN2D; I79_013051; Kiaa4197; mKIAA4197; Nav1.7; NE-NA; NENA; PN1; Scn2a; SCN9A; SFNP
UniProt ID: (Human) Q15858, (Mouse) Q62205, (Rat) O08562
Entrez Gene ID: (Human) 6335, (Chinese hamster) 100751370, (Mouse) 20274, (Rat) 78956
action potential
ion transport
sodium ion transport
inflammatory response
circadian rhythm
response to toxic substance
post-embryonic development
neuronal action potential
sensory perception of pain
ion transmembrane transport
sodium ion transmembrane transport
behavioral response to pain
detection of temperature stimulus involved in sensory perception of pain
detection of mechanical stimulus involved in sensory perception
transmembrane transport
cardiac muscle cell action potential involved in contraction
action potential propagation
transport
regulation of ion transmembrane transport
membrane depolarization during action potential
rhythmic excitation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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