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Invitrogen
CACNB2 Monoclonal Antibody (N8b/1), APC
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FIGURE: 1 / 4
CACNB2 Antibody (MA5-45450) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with Cav beta 2 monoclonal antibody (Product # MA5-45450) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav beta 2 antibody (D) Compo... View More
Product Details
MA5-45450
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N8b/1
Immunogen
Synthetic peptide amino acids 189-205 of rat CavBeta2
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45450 was sufficient for detection of Cav beta2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody|Detects approximately 78 kDa. No cross reactivity against Cav beta 1, Cav beta 3, Cav beta 4.
Target Information
The expression of multiple classes of voltage dependent calcium channels (VDCCs) allows neurons to tailor calcium signaling to functionally discrete cellular regions. Although N-Type VDCCs exist before birth which is consistent with a role in migration, most N-Type VDCCs subunit expression is postnatal and is important in the genesis of synaptic transmission in discrete hippocampal fields.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CAB1; CAB2; CACNLB1; Calcium channel voltage-dependent subunit beta 2; calcium channel, voltage-dependent, beta 2 subunit; CCHLB1; Lambert-Eaton myasthenic syndrome antigen B; MGC138502; MGC138504; MGC41896; myasthenic (Lambert-Eaton) syndrome antigen B; MYSB; unnamed protein product; Voltage-dependent L-type calcium channel subunit beta-2; voltage-gated calcium channel beta2 subunit transcript variant X13
Gene Aliases: AW060387; CAB2; CACNB2; CACNLB2; CAVB2; Cavbeta2; Cchb2; MYSB
UniProt ID: (Human) Q08289, (Mouse) Q8CC27, (Rat) Q8VGC3
Entrez Gene ID: (Human) 783, (Mouse) 12296, (Rat) 116600
voltage-gated calcium channel activity
calcium channel activity
protein binding
high voltage-gated calcium channel activity
actin filament binding
voltage-gated calcium channel activity involved in cardiac muscle cell action potential
voltage-gated calcium channel activity involved in AV node cell action potential
voltage-gated calcium channel activity involved in regulation of presynaptic cytosolic calcium levels
voltage-gated ion channel activity
calcium channel regulator activity
protein kinase binding
protein domain specific binding
phosphoprotein binding
voltage-gated ion channel
ion transport
calcium ion transport
chemical synaptic transmission
neuromuscular junction development
visual perception
ion transmembrane transport
positive regulation of muscle contraction
positive regulation of calcium ion transport
calcium ion import
calcium ion transmembrane transport
protein localization to plasma membrane
membrane depolarization during AV node cell action potential
regulation of heart rate by cardiac conduction
membrane depolarization during atrial cardiac muscle cell action potential
regulation of presynaptic cytosolic calcium ion concentration
positive regulation of calcium ion transmembrane transport via high voltage-gated calcium channel
transport
regulation of ion transmembrane transport
regulation of voltage-gated calcium channel activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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