Search
Invitrogen
SLC5A5 Monoclonal Antibody (FP5), PerCP
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 5
SLC5A5 Antibody (MA5-45494) in IHC (PFA)
Immunohistochemistry analysis using Thyroid. Fixation involved 10% formalin solution for 12-24 hours at RT. Samples were incubated with Sodium Iodide Symporter monoclonal antibody (Product # MA5-45494) at 1:1,000 for 1 hour at RT, followed by HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain used was Mayer Hematoxylin (p... View More
Product Details
MA5-45494
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
FP5
Immunogen
Mannose binding protein hNIS fusion (AA468-643)
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45494 was sufficient for detection of hNIS in 20 µg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at approximately 30 kDa, and approximately 15kDa.
Target Information
Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane bound glycoprotein with 13 membrane spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na(+)/I(-)-symporter; Na+/I- symporter; Natrium iodide transporter; Sodium-iodide symporter; Sodium/iodide cotransporter; solute carrier family 5 (sodium iodide symporter), member 5; solute carrier family 5 (sodium/iodide cotransporter), member 5; Solute carrier family 5 member 5; solute carrier family 5, member 5
Gene Aliases: NIS; SLC5A5; TDH1
UniProt ID: (Human) Q92911, (Rat) Q63008
Entrez Gene ID: (Human) 6528, (Rat) 114613, (Mouse) 114479
protein binding
sodium:iodide symporter activity
iodide transmembrane transporter activity
symporter activity
solute:sodium symporter activity
monovalent anion:sodium symporter activity
transmembrane transporter activity
protein homodimerization activity
metal ion binding
transporter activity
secondary carrier transporter
thyroid hormone generation
ion transport
sodium ion transport
iodide transport
sodium ion transmembrane transport
transmembrane transport
cellular response to cAMP
cellular response to gonadotropin stimulus
transport across blood-brain barrier
iodide transmembrane transport
cellular response to forskolin
cellular response to Thyroid stimulating hormone
anion transport
anion transmembrane transport
transport
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support