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Invitrogen
VAChT Monoclonal Antibody (N6/38), FITC
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FIGURE: 1 / 3
VAChT Antibody (MA5-45500) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with VAChT monoclonal antibody (Product # MA5-45500) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin st... View More
Product Details
MA5-45500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N6/38
Immunogen
Synthetic peptide amino acids 521-532 of human VAChT
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
9.09mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A dilution of 1:50-1:200 of MA5-45500 was sufficient for detection of VAChT Transporter in rat brain using immunohistochemistry analysis and goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 56kDa.
This antibody was formerly sold as clone S6-38.
Target Information
This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC12716; solute carrier family 18 (vesicular acetylcholine transporter), member 3; solute carrier family 18 (vesicular acetylcholine), member 3; solute carrier family 18 (vesicular monoamine) member 3; Solute carrier family 18 member 3; solute carrier family 18, member 3; unnamed protein product; VAChT; Vesicular acetylcholine transporter
Gene Aliases: CMS21; rVAT; SLC18A3; VACHT; VAT
UniProt ID: (Human) Q16572, (Rat) Q62666
Entrez Gene ID: (Human) 6572, (Mouse) 20508, (Rat) 60422
neurotransmitter transport
chemical synaptic transmission
positive regulation of acetylcholine secretion, neurotransmission
serotonin uptake
acetylcholine uptake
transmembrane transport
positive regulation of long-term synaptic potentiation
hydrogen ion transmembrane transport
positive regulation of neuromuscular junction development
cation transport
organic cation transport
monoamine transport
ammonium transmembrane transport
cation transmembrane transport
acetate ester transport
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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