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MMP9 Monoclonal Antibody (L51/82), FITC

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MMP9 Monoclonal Antibody (L51/82), FITC

Product Details

MA5-45512

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

L51/82

Immunogen

Fusion protein amino acids 1-708 (full length) of rat MMP9
3D Epitope / Immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein G

Storage buffer

9.09mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931966

Product Specific Information

1 µg/mL of MA5-45512 was sufficient for detection of MMP9 in 20 µg of COS-1 cells (lysate) transfected with human MMP9 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Detects approximately 92kDa and approximately 82kDa (pro and active forms).

This antibody was formerly sold as clone S51-82.

Target Information

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 92 kDa gelatinase; 92 kDa type IV collagenase; 92-kDa type IV collagenase; 92kD gelatinase; 92kD type IV collagenase; CLG4B; collagenase type IVB; gelatinase B; Gelatinase-B; GELB; macrophage gelatinase; matrix metallo protease; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase; matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix metalloproteinase9; MMP; MMP-9; MMP9; MMPs; type V collagenase; unnamed protein product

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Gene Aliases: B/MMP9; CLG4B; Gel B; GELB; MANDP2; MMP-9; pro-MMP-9

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UniProt ID: (Human) P14780, (Rat) P50282, (Mouse) P41245

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Entrez Gene ID: (Human) 4318, (Rat) 81687, (Mouse) 17395

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Function(s)
endopeptidase activity metalloendopeptidase activity serine-type endopeptidase activity protein binding collagen binding peptidase activity metallopeptidase activity zinc ion binding identical protein binding fibronectin binding macromolecular complex binding
Process(es)
skeletal system development ossification response to hypoxia kidney development positive regulation of protein phosphorylation positive regulation of leukocyte migration proteolysis response to oxidative stress heart development embryo implantation parturition response to nutrient response to xenobiotic stimulus response to mechanical stimulus response to high light intensity cell migration extracellular matrix disassembly protein catabolic process extracellular matrix organization positive regulation of cell migration collagen catabolic process positive regulation of synaptic plasticity response to estradiol response to lipopolysaccharide response to retinoic acid response to vitamin A response to tumor necrosis factor response to nicotine endodermal cell differentiation positive regulation of apoptotic process negative regulation of apoptotic process response to ethanol positive regulation of epidermal growth factor receptor signaling pathway positive regulation of angiogenesis negative regulation of fibroblast proliferation macrophage chemotaxis tissue remodeling regulation of multicellular organismal process positive regulation of keratinocyte migration response to hyperoxia cellular response to lipopolysaccharide cellular response to iron(III) ion cellular response to cytokine stimulus cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to low-density lipoprotein particle stimulus cellular response to cell-matrix adhesion cellular response to UV-A positive regulation of release of cytochrome c from mitochondria modification of synaptic structure, modulating synaptic transmission regulation of postsynaptic neurotransmitter receptor diffusion trapping negative regulation of cation transmembrane transport response to amyloid-beta positive regulation of vascular smooth muscle cell proliferation negative regulation of epithelial cell differentiation involved in kidney development negative regulation of intrinsic apoptotic signaling pathway macrophage differentiation positive regulation of DNA binding ephrin receptor signaling pathway regulation of neuroinflammatory response
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