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Invitrogen
SLC38A1 Monoclonal Antibody (N104/32), PE
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FIGURE: 1 / 3
SLC38A1 Antibody (MA5-45532) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with SNAT1 monoclonal antibody (Product # MA5-45532) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin sta... View More
Product Details
MA5-45532
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N104/32
Immunogen
Fusion protein amino acids 1- 63 of rat SNAT1
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45532 was sufficient for detection of SNAT1 in 20 µg of lysates from neocortical neurons cultured under amino acid starvation conditions and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 50kDa.
This antibody was formerly sold as clone S104-32.
Target Information
Amino acid transporters play essential roles in the uptake of nutrients, production of energy, chemical metabolism, detoxification, and neurotransmitter cycling. SLC38A1 is an important transporter of glutamine, an intermediate in the detoxification of ammonia and the production of urea. Glutamine serves as a precursor for the synaptic transmitter, glutamate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amino acid transporter A1; amino acid transporter system A1; ATA1; Glutamine transporter; MNat2; N-system amino acid transporter 2; rATA1; S38A1; SNAT 1; Sodium-coupled neutral amino acid symporter 1; sodium-coupled neutral amino acid transporter 1; Solute carrier family 38 member 1; System A amino acid transporter 1; System A transporter 2; System N amino acid transporter 1; unnamed protein product
Gene Aliases: AA408026; AA409865; AL022800; ATA1; AU015942; GlnT; NAT2; Sa2; SAT1; SLC38A1; SNAT1
UniProt ID: (Human) Q9H2H9, (Rat) Q9JM15, (Mouse) Q8K2P7
Entrez Gene ID: (Human) 81539, (Rat) 170567, (Mouse) 105727
sodium:amino acid symporter activity
neutral amino acid:sodium symporter activity
proline:sodium symporter activity
protein binding
amino acid transmembrane transporter activity
neutral amino acid transmembrane transporter activity
L-amino acid transmembrane transporter activity
L-glutamine transmembrane transporter activity
symporter activity
glycine:sodium symporter activity
alanine:sodium symporter activity
amino acid transporter
neurotransmitter uptake
amino acid transmembrane transport
ion transport
sodium ion transport
amino acid transport
glutamine transport
female pregnancy
gamma-aminobutyric acid biosynthetic process
neutral amino acid transport
glycine transport
regulation of synaptic transmission, GABAergic
alanine transport
sodium ion transmembrane transport
amino acid import
regulation of synaptic plasticity
transport across blood-brain barrier
L-alpha-amino acid transmembrane transport
L-glutamine import across plasma membrane
cation transmembrane transport
transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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