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Invitrogen

EAAC1 Monoclonal Antibody (N180/41), PerCP

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EAAC1 Monoclonal Antibody (N180/41), PerCP

Product Details

MA5-45549

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N180/41

Immunogen

Fusion protein amino acids 1-524 (full length) of human EAAC1
View immunogen

Conjugate

PerCP PerCP PerCP

Excitation/Emission Max

482/675 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932003

Product Specific Information

5 µg of MA5-45549 was sufficient for immunoprecipitation of EAAC1 from 50 µg of rat brain lysate.|Detects approximately 60kDa.

This antibody was formerly sold as clone S180-41.

Target Information

L-glutamate is the major excitatory neurotransmitter in mammalian central nervous systems. To maintain glutamate levels below excitoxic levels, excess glutamate at excitatory synaptic clefts is removed by ion-coupled glutamate transporters. Four glutamate transporters have been identified: the sodium-dependent GLAST-1, GLT-1, EAAC-1 and the chloride-dependent EAAT-4. EAAC-1 is expressed in the CNS and also found in kidney and small intestine. Structural features of glutamate transporters are believed to include eight membrane-spanning alpha-helices and a loop-pore structure which is unique among secondary transporters but may resemble loop-pores found in ion channels. A second distinctive structural feature is the presence of a highly amphipathic membrane-spanning helix that provides a hydrophilic path through the membrane. Mice with homozygous deletions of the EAAC-1 transporter gene develop dicarboxylic aminoaciduria and display reduced spontaneous locomotor activity although no neurodegeneration was observed over a period of 12 months.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: excitatory amino acid carrier 1; Excitatory amino acid transporter 3; excitatory amino acid transporter-3; Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter; Sodium-dependent glutamate/aspartate transporter 3; solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1; Solute carrier family 1 A1 (brain glutamate transporter); Solute carrier family 1 member 1; solute carrier family 1, member 1; unnamed protein product

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Gene Aliases: DCBXA; EAAC1; EAAT3; HEAAC1; REAAC1; SCZD18; SLC1A1

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UniProt ID: (Human) P43005, (Rat) P51907

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Entrez Gene ID: (Human) 6505, (Rat) 25550

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Function(s)
anion channel activity L-glutamate transmembrane transporter activity high-affinity glutamate transmembrane transporter activity protein binding chloride transmembrane transporter activity L-aspartate transmembrane transporter activity symporter activity glutamate:sodium symporter activity cysteine transmembrane transporter activity identical protein binding metal ion binding D-aspartate transmembrane transporter activity glutamate binding sodium:dicarboxylate symporter activity primary active transporter
Process(es)
behavioral fear response response to amphetamine regulation of heart rate glutathione metabolic process glutathione biosynthetic process superoxide metabolic process ion transport neurotransmitter transport amino acid transport cellular zinc ion homeostasis dopamine receptor signaling pathway glutamate receptor signaling pathway chemical synaptic transmission brain development learning or memory memory grooming behavior locomotory behavior response to xenobiotic stimulus positive regulation of heart rate gene expression retina layer formation L-glutamate transport cytokine-mediated signaling pathway neurogenesis adult behavior cellular response to oxidative stress maintenance of blood-brain barrier response to decreased oxygen levels dopamine metabolic process cysteine transport response to morphine negative regulation of neuron apoptotic process establishment of protein localization blood vessel morphogenesis response to axon injury synapse organization neuron apoptotic process L-glutamate import righting reflex retina development in camera-type eye heart contraction long-term synaptic potentiation motor behavior transepithelial transport D-aspartate transmembrane transport L-aspartate transmembrane transport D-aspartate import cellular response to ammonium ion cellular response to mercury ion cellular response to cocaine zinc II ion transmembrane transport response to anesthetic regulation of protein targeting to membrane glutamate homeostasis motor neuron apoptotic process import into cell L-glutamate import across plasma membrane neurotransmitter receptor transport to plasma membrane postsynaptic modulation of chemical synaptic transmission L-aspartate import across plasma membrane transport across blood-brain barrier chloride transmembrane transport cysteine transmembrane transport cellular response to bisphenol A conditioned place preference sodium ion transmembrane transport protein homooligomerization L-glutamate transmembrane transport
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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