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Invitrogen
mGluR1/mGluR5 Monoclonal Antibody (N75/33), PerCP
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FIGURE: 1 / 3
mGluR1/mGluR5 Antibody (MA5-45552) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with mGluR1/5 monoclonal antibody (Product # MA5-45552) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45552
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N75/33
Immunogen
Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b
Conjugate
PerCP
PerCP
PerCP
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45552 was sufficient for detection of mGluR1/5 glutamate receptor in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 130kDa (both mGluR1 and mGluR5).
This antibody was formerly sold as clone S75-33.
Target Information
may play a role in synaptic transmission; may interact with mGluR1, another group I metabotropic glutamate receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G protein coupled receptor family C group 1 member A; G protein coupled receptor, family C, group 1, member A; G protein coupled receptor, family C, group 1, member E; G protein-coupled receptor GRM5; G protein-coupled receptor, family C, group 1, member A; G protein-coupled receptor, family C, group 1, member E; glutamate receptor; metabotropic glutamate receptor (mGluR5); Metabotropic glutamate receptor 1; Metabotropic glutamate receptor 5; metabotropic glutamate receptor 5b; metabotropic glutamate receptor subtype 5b; metabotropic glutamate receptor type 5; mGluR1; mGluR5; mGlurR1a
Gene Aliases: 4930455H15Rik; 6430542K11Rik; AI850523; ENSMUSG00000075319; Glu5R; Gm10828; GPRC1A; GPRC1E; GRM1; GRM5; MGLUR1; MGLUR5; mGluR5b; nmf373; rcw; wobl
UniProt ID: (Mouse) P97772, (Rat) P23385, (Rat) P31424, (Mouse) Q3UVX5
Entrez Gene ID: (Mouse) 14816, (Rat) 24414, (Rat) 24418, (Mouse) 108071
PLC activating G-protein coupled glutamate receptor activity
signal transducer activity
G-protein coupled receptor activity
protein binding
glutamate receptor activity
estrogen receptor binding
identical protein binding
calmodulin binding
PDZ domain binding
A2A adenosine receptor binding
G-protein coupled receptor
activation of MAPKK activity
activation of MAPK activity
signal transduction
G-protein coupled receptor signaling pathway
protein kinase C-activating G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled glutamate receptor signaling pathway
G-protein coupled glutamate receptor signaling pathway
locomotory behavior
sensory perception of pain
calcium-mediated signaling
dimeric G-protein coupled receptor signaling pathway
regulation of MAPK cascade
positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway
regulation of sensory perception of pain
regulation of synaptic transmission, glutamatergic
cellular response to electrical stimulus
chemical synaptic transmission
regulation of glutamate secretion
membrane depolarization
synaptic transmission, GABAergic
activation of MAPKKK activity
desensitization of G-protein coupled receptor protein signaling pathway
regulation of transcription, DNA-templated
protein phosphorylation
learning
negative regulation of locomotion
phosphatidylinositol-mediated signaling
regulation of long-term neuronal synaptic plasticity
positive regulation of long-term neuronal synaptic plasticity
cognition
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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