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Invitrogen
Synaptotagmin 7 Monoclonal Antibody (N275/14), PE
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FIGURE: 1 / 3
Synaptotagmin 7 Antibody (MA5-45591) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Synaptotagmin-7 monoclonal antibody (Product # MA5-45591) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45591
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
N275/14
Immunogen
Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45591 was sufficient for detection of Synaptotagmin-7 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 45kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at approximately 65kD.
This antibody was formerly sold as clone S275-14.
Target Information
This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. A similar protein in rodents mediates hormone secretion and lysosome exocytosis. In humans, expression of this gene has been associated with prostate cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alternatively spliced; long form; prostate cancer-associated protein 7; protein Syt7; short form; similar to rat synaptotagmin VII; synaptotagmin VII; SYT-7; sytVII; truncated form 1; truncated form 2; unnamed protein product
Gene Aliases: B230112P13Rik; IPCA-7; IPCA7; PCANAP7; SYT-VII; SYTVII
UniProt ID: (Human) O43581, (Mouse) Q9R0N7, (Rat) Q62747
Entrez Gene ID: (Human) 9066, (Mouse) 54525, (Rat) 59267
plasma membrane repair
vesicle fusion
phagocytosis
vesicle-mediated transport
calcium ion regulated exocytosis
regulation of calcium ion-dependent exocytosis
regulation of endocytosis
synaptic vesicle recycling
positive regulation of calcium ion-dependent exocytosis
regulation of bone remodeling
calcium ion-regulated exocytosis of neurotransmitter
regulation of phagocytosis
regulation of insulin secretion
positive regulation of transport
regulation of glucagon secretion
vesicle-mediated cholesterol transport
phagosome-lysosome fusion
calcium-dependent activation of synaptic vesicle fusion
regulation of synaptic vesicle endocytosis
short-term synaptic potentiation
calcium ion regulated lysosome exocytosis
regulation of dopamine secretion
insulin secretion
detection of calcium ion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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