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Invitrogen
ASIC1 Monoclonal Antibody (N271/44), PE
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FIGURE: 1 / 3
ASIC1 Antibody (MA5-45595) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with ASIC1 monoclonal antibody (Product # MA5-45595) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45595
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N271/44
Immunogen
Fusion protein amino acids 460-526 (Cytoplasmic C-terminus) of mouse ASIC1
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45595 was sufficient for detection of ASIC1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 60kDa.
This antibody was formerly sold as clone S271-44.
Target Information
This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. ASIC1 is permeable for Ca(2+), Li(+) and K(+) and is expressed in dorsal root ganglia, sciatic nerves, olfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum. It is diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACCN2 variant 3; acid sensing ion channel; Acid-sensing ion channel; Acid-sensing ion channel 1; Acid-sensing ionic channel; Amiloride-sensitive cation channel 2, neuronal; ASIC; ASIC1; BNaC2; Brain sodium channel 2; degenerin 2; hBNaC2; proton gated cation channel ASIC1
Gene Aliases: ACCN2; AI843610; ASIC; ASIC1; ASIC1a; B530003N02Rik; BNAC2
UniProt ID: (Mouse) Q6NXK8, (Rat) P55926
Entrez Gene ID: (Mouse) 11419, (Rat) 79123
transport
ion transport
cation transport
sodium ion transport
calcium ion transport
memory
associative learning
response to acidic pH
monovalent inorganic cation transport
ion transmembrane transport
sodium ion transmembrane transport
regulation of membrane potential
negative regulation of neurotransmitter secretion
sensory perception of sour taste
calcium ion transmembrane transport
cellular response to pH
sensory perception of pain
protein homotrimerization
cation transmembrane transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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