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Invitrogen
alpha-2c Adrenergic Receptor Monoclonal Antibody (N330A/80), PE
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FIGURE: 1 / 3
alpha-2c Adrenergic Receptor Antibody (MA5-45618) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Alpha-2C Adrenergic Receptor monoclonal antibody (Product # MA5-45618) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at ... View More
Product Details
MA5-45618
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N330A/80
Immunogen
Synthetic peptide amino acids 442- 462 (QDFRRSFKHILFRRRRRGFRQ, cytoplasmic C-terminus) of human Alpha-2C adrenergic receptor
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45618 was sufficient for detection of Alpha2C Adrenergic Receptor in 20 µg of COS cells transiently transfected with HA-tagged Alpha- lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |Detects 50kDa or larger (possibly due to dimerization). Does not cross-react with other adrenergic receptors.
This antibody was formerly sold as clone S330A-80.
Target Information
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. The mouse studies revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes the alpha2C subtype, which contains no introns in either its coding or untranslated sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adra 2c; adrenergic, alpha-2C-, receptor; Alpha 2CAR; Alpha-2 adrenergic receptor subtype C4; Alpha-2C adrenergic receptor; Alpha-2C adrenoceptor; Alpha-2C adrenoreceptor; Alpha-2C-AR; alpha-2CAR; alpha2 AR C4; alpha2 C4; alpha2-AR-C4; kidney alpha-2-adrenergic receptor; subtype alpha2-C4
Gene Aliases: [a]2C; Adra-2c; ADRA2C; ADRA2L2; ADRA2RL2; ADRARL2; alpha2-C4; alpha2C; ALPHA2CAR
UniProt ID: (Human) P18825, (Mouse) Q01337
Entrez Gene ID: (Human) 152, (Mouse) 11553
G-protein coupled receptor activity
adrenergic receptor activity
alpha2-adrenergic receptor activity
guanyl-nucleotide exchange factor activity
protein binding
alpha-2A adrenergic receptor binding
protein homodimerization activity
protein heterodimerization activity
epinephrine binding
signal transducer activity
G-protein coupled receptor
regulation of smooth muscle contraction
signal transduction
epidermal growth factor receptor signaling pathway
G-protein coupled receptor signaling pathway
cell-cell signaling
negative regulation of norepinephrine secretion
regulation of vasoconstriction
platelet activation
negative regulation of epinephrine secretion
positive regulation of MAPK cascade
positive regulation of neuron differentiation
negative regulation of insulin secretion
positive regulation of protein kinase B signaling
adrenergic receptor signaling pathway
negative regulation of adenylate cyclase activity
female pregnancy
activation of protein kinase B activity
epidermal growth factor-activated receptor transactivation by G-protein coupled receptor signaling pathway
positive regulation of vasoconstriction
regulation of sensory perception of pain
negative regulation of insulin secretion involved in cellular response to glucose stimulus
negative regulation of uterine smooth muscle contraction
adenylate cyclase-inhibiting adrenergic receptor signaling pathway
activation of MAPK activity by adrenergic receptor signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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