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Invitrogen
PTPRF Monoclonal Antibody (N165/38), PerCP
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FIGURE: 1 / 3
PTPRF Antibody (MA5-45639) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with LAR/PTPRF monoclonal antibody (Product # MA5-45639) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stai... View More
Product Details
MA5-45639
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
N165/38
Immunogen
Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR.
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
97% identical in both rat and mouse. >80% identity with PTPRD and PTPRS. >50% identity with PTPRM and PTPRK.
1 µg/mL of MA5-45639 was sufficient for detection of LAR/PTPRF in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 85kDa (full length protein is 210 kDa - smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains.
This antibody was formerly sold as clone S165-38.
Target Information
Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase).The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ43335; FLJ45062; FLJ45567; LAR; LAR protein; LCA homolog; LCA-homolog; leukocyte antigen-related (LAR) PTP receptor; leukocyte antigen-related tyrosine phosphatase; Leukocyte common antigen related; protein tyrosine phosphatase receptor-type F; protein tyrosine phosphatase, receptor type, F polypeptide; PTPRF protein; receptor-linked protein-tyrosine phosphatase LAR; Receptor-type tyrosine-protein phosphatase F; unnamed protein product
Gene Aliases: AA591035; BNAH2; LAR; LARFN5C; LARS; PTPRF; RPTP-LAR
UniProt ID: (Human) P10586, (Rat) Q64604, (Mouse) A2A8L5
Entrez Gene ID: (Human) 5792, (Rat) 360406, (Mouse) 19268
phosphoprotein phosphatase activity
protein tyrosine phosphatase activity
transmembrane receptor protein tyrosine phosphatase activity
heparin binding
hydrolase activity
chondroitin sulfate proteoglycan binding
macromolecular complex binding
cell adhesion molecule binding
insulin receptor binding
receptor tyrosine kinase binding
protein complex binding
phosphate ion binding
phosphatase activity
protein phosphatase
protein modifying enzyme
cell adhesion
signal transduction
transmembrane receptor protein tyrosine phosphatase signaling pathway
nervous system development
regulation of neuron projection development
cell migration
neuron projection regeneration
peptidyl-tyrosine dephosphorylation
regulation of axon regeneration
synaptic membrane adhesion
negative regulation of receptor binding
negative regulation of cytokine-mediated signaling pathway
protein dephosphorylation
homophilic cell adhesion via plasma membrane adhesion molecules
negative regulation of cell proliferation
negative regulation of cell projection organization
negative regulation of epidermal growth factor receptor signaling pathway
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
negative regulation of insulin receptor signaling pathway
positive regulation of dendrite morphogenesis
regulation of synapse structure or activity
negative regulation of neurotrophin TRK receptor signaling pathway
dephosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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