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Invitrogen
Protocadherin Gamma Monoclonal Antibody (N159/5), APC
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FIGURE: 1 / 3
Protocadherin Gamma Antibody (MA5-45658) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Protocadherin Gamma (pan) monoclonal antibody (Product # MA5-45658) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1... View More
Product Details
MA5-45658
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N159/5
Immunogen
Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941).
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45658 was sufficient for detection of Protocadherin gamma (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 100kDa. Cross-reacts with all Gamma-protocadherin-A, -B and -C proteins.
This antibody was formerly sold as clone S159-5.
Target Information
The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3' RACE product; cadherin 20; cadherin 21; cadherin ME3; cadherin ME6; Cadherin-20; Cadherin-21; cadherin-like 2; fibroblast cadherin FIB2; fibroblast cadherin FIB3; Fibroblast cadherin-2; Fibroblast cadherin-3; Pan-Gamma-Protocadherin-A; PC-43; PCDH gamma A; PCDH-gamma-A; PCDH-gamma-A1; PCDH-gamma-A10; PCDH-gamma-A11; PCDH-gamma-A12; PCDH-gamma-A2; PCDH-gamma-A3; PCDH-gamma-A4; PCDH-gamma-A5; PCDH-gamma-A6; PCDH-gamma-A7; PCDH-gamma-A8; PCDH-gamma-A9; PCDH-gamma-B1; PCDH-gamma-B2; PCDH-gamma-B3; PCDH-gamma-B4; PCDH-gamma-B5; PCDH-gamma-B6; PCDH-gamma-B7; PCDH-gamma-C3; PCDH-gamma-C4; PCDH-gamma-C5; PCDHGA; pcdhga4; protocadherin 13; protocadherin 2; protocadherin 43; protocadherin gamma a1; protocadherin gamma A12; protocadherin gamma a2; protocadherin gamma B2; protocadherin gamma B6; protocadherin gamma b7; protocadherin gamma C-VI; protocadherin gamma c5; protocadherin gamma subfamily A, 10; protocadherin gamma subfamily A, 12; protocadherin gamma subfamily A, 3; protocadherin gamma subfamily A, 4; protocadherin gamma subfamily B, 1, isoform 2; protocadherin gamma subfamily B, 5; protocadherin gamma subfamily B, 7; protocadherin gamma subfamily C, 3; Protocadherin gamma-A1; Protocadherin gamma-A10; Protocadherin gamma-A11; Protocadherin gamma-A12; Protocadherin gamma-A2; Protocadherin gamma-A3; Protocadherin gamma-A4; Protocadherin gamma-A5; Protocadherin gamma-A6; protocadherin gamma-A6-like; Protocadherin gamma-A7; Protocadherin gamma-A8; Protocadherin gamma-A9; Protocadherin gamma-B1; Protocadherin gamma-B2; Protocadherin gamma-B3; Protocadherin gamma-B4; protocadherin gamma-B4-like; Protocadherin gamma-B5; Protocadherin gamma-B6; protocadherin gamma-B6-like; Protocadherin gamma-B7; Protocadherin gamma-C3; Protocadherin gamma-C4; Protocadherin gamma-C5; Protocadherin-2; Protocadherin-43
Gene Aliases: CDH-GAMMA-A5; CDH20; CDH21; FIB2; FIB3; KIAA0327; KIAA0588; ME3; ME6; mKIAA0588; mKIAA4054; pc2c; PC43; PCDH-GAMMA-A1; PCDH-GAMMA-A10; PCDH-GAMMA-A11; PCDH-GAMMA-A12; PCDH-GAMMA-A2; PCDH-GAMMA-A3; PCDH-GAMMA-A4; PCDH-GAMMA-A5; PCDH-GAMMA-A6; PCDH-GAMMA-A7; PCDH-GAMMA-A8; PCDH-GAMMA-A9; PCDH-GAMMA-B1; PCDH-GAMMA-B2; PCDH-GAMMA-B3; PCDH-GAMMA-B4; PCDH-GAMMA-B5; PCDH-GAMMA-B6; PCDH-GAMMA-B7; PCDH-GAMMA-C3; PCDH-GAMMA-C4; PCDH-GAMMA-C5; Pcdh13; PCDH2; PCDHGA1; PCDHGA10; Pcdhga10temp; PCDHGA11; PCDHGA12; PCDHGA2; PCDHGA3; PCDHGA4; PCDHGA5; PCDHGA6; PCDHGA7; PCDHGA8; PCDHGA9; PCDHGB1; PCDHGB2; PCDHGB3; PCDHGB4; PCDHGB5; PCDHGB6; PCDHGB7; PCDHGC3; PCDHGC4; PCDHGC5; UNQ371/PRO707
UniProt ID: (Human) Q9Y5H4, (Human) Q9Y5H3, (Human) Q9Y5H2, (Human) O60330, (Human) Q9Y5H1, (Human) Q9Y5H0, (Human) Q9Y5G9, (Human) Q9Y5G8, (Human) Q9Y5G7, (Human) Q9Y5G6, (Human) Q9Y5G5, (Human) Q9Y5G4, (Human) Q9Y5G3, (Human) Q9Y5G2, (Human) Q9Y5G1, (Human) O15099, (Human) Q9Y5G0, (Human) Q9Y5F9, (Human) Q9Y5F8, (Human) Q9UN70, (Human) Q9Y5F7, (Human) Q9Y5F6, (Mouse) Q91XY4
Entrez Gene ID: (Rat) 102552497, (Rat) 102552705, (Rat) 102552796, (Human) 56114, (Human) 56106, (Human) 56105, (Human) 26025, (Human) 56113, (Human) 56112, (Human) 56111, (Human) 56110, (Human) 56109, (Human) 56108, (Human) 9708, (Human) 56107, (Human) 56104, (Human) 56103, (Human) 56102, (Human) 8641, (Human) 56101, (Human) 56100, (Human) 56099, (Human) 5098, (Human) 56098, (Human) 56097, (Rat) 553129, (Mouse) 93709, (Rat) 498849, (Mouse) 93722, (Rat) 252897, (Mouse) 93723, (Mouse) 93724, (Rat) 498850, (Rat) 498846, (Mouse) 93710, (Mouse) 93711, (Rat) 498847, (Mouse) 93712, (Rat) 252894, (Rat) 291637, (Mouse) 93713, (Mouse) 93714, (Mouse) 93715, (Rat) 291635, (Rat) 364843, (Mouse) 93716, (Rat) 252895, (Mouse) 93717, (Mouse) 93699, (Mouse) 93700, (Mouse) 93701, (Mouse) 93702, (Rat) 680448, (Mouse) 93703, (Mouse) 93704, (Rat) 364844, (Mouse) 93706, (Rat) 116782, (Mouse) 93707, (Mouse) 93708, (Rat) 641525
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