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Neuroligin 1 Monoclonal Antibody (N97A/31), FITC

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Neuroligin 1 Monoclonal Antibody (N97A/31), FITC

Product Details

MA5-45686

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N97A/31

Immunogen

Fusion protein amino acids 718-843 (cytoplasmic C-terminus) of rat Neuroligin-1.
3D Epitope / Immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932140

Product Specific Information

Mouse: 99% identity (125/126 amino acids identical). Human: 99% identity (125/126 amino acids identical) >40% identity with Neuroligin-2 and -3.

2 µg/mL of MA5-45686 was sufficient for detection of Neuroligin-1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 120kDa. Does not cross-react with other Neuroligins.

This antibody was formerly sold as clone S97A-31.

Target Information

This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC45115; neuroligin I; NLG 1; unnamed protein product

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Gene Aliases: 6330415N05Rik; mKIAA1070; NL1; NLG1

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UniProt ID: (Human) Q8N2Q7, (Mouse) Q99K10, (Rat) Q62765

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Entrez Gene ID: (Human) 22871, (Mouse) 192167, (Rat) 116647

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Function(s)
beta-amyloid binding PDZ domain binding signaling receptor activity neurexin family protein binding identical protein binding cell adhesion molecule binding scaffold protein binding cell adhesion mediator activity protein binding macromolecular complex binding
Process(es)
regulation of respiratory gaseous exchange by neurological system process protein targeting heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules neuron cell-cell adhesion nervous system development synapse assembly regulation of signal transduction positive regulation of circadian sleep/wake cycle, wakefulness positive regulation of neuron projection development calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules neuron projection development regulation of neurological system process positive regulation of synaptic transmission, GABAergic protein localization to synapse regulation of membrane potential establishment of protein localization regulation of neuron differentiation synaptic vesicle transport cytoskeletal matrix organization at active zone neuron projection morphogenesis modulation of synaptic transmission synapse organization positive regulation of multicellular organismal process positive regulation of filopodium assembly positive regulation of synapse assembly positive regulation of synaptic transmission, glutamatergic synapse maturation inhibitory postsynaptic potential long-term synaptic potentiation positive regulation of dendritic spine development negative regulation of dendritic spine morphogenesis cellular response to calcium ion membrane assembly terminal button organization cell-cell adhesion involved in synapse maturation synaptic vesicle clustering postsynaptic membrane assembly presynaptic membrane assembly AMPA glutamate receptor clustering NMDA glutamate receptor clustering neurexin clustering involved in presynaptic membrane assembly postsynaptic density protein 95 clustering receptor localization to synapse postsynaptic specialization assembly NMDA selective glutamate receptor signaling pathway AMPA selective glutamate receptor signaling pathway regulation of postsynaptic density assembly regulation of presynapse organization maintenance of synapse structure synaptic membrane adhesion neuron projection arborization positive regulation of ruffle assembly positive regulation of neuromuscular synaptic transmission positive regulation of synaptic vesicle endocytosis positive regulation of protein localization to synapse positive regulation of intracellular signal transduction excitatory synapse assembly positive regulation of presynaptic active zone assembly positive regulation of synaptic vesicle exocytosis regulation of N-methyl-D-aspartate selective glutamate receptor activity positive regulation of excitatory postsynaptic potential positive regulation of synaptic vesicle clustering neuronal ion channel clustering neuronal signal transduction presynapse assembly
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