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NLGN4X (Neuroligin-4) Monoclonal Antibody (N98/7), PE
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FIGURE: 1 / 1
NLGN4X (Neuroligin-4) Antibody (MA5-45696) in WB
Western Blot analysis of Mouse Brain Membrane showing detection of ~125-130 kDa Neuroligin 4 protein. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Membrane. Load: 15 µg. Blocking: 2% BSA and 2% Skim Milk in 1X TBST. Samples were incubated with Neuroligin 4 monoclonal antibody (Product # MA5-45696) at 1:1,000 for 16 hours at 4°C, followed by Goat Anti-Mouse IgG: HRP at 1:2,000 for 60 min at RT. ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45696
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N98/7
Immunogen
Fusion protein amino acids 782-945 (intracellular C-terminus) of mouse Neuroligin 4.
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
~45% identity with Neuroligin-1, -2 and -3.
1 µg/mL of MA5-45696 was sufficient for detection of Neuroligin 4 in 20 µg of mouse brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 125-130kDa. Does not cross-react with other Neuroligins.
This antibody was formerly sold as clone S98-7.
Target Information
Neuroligin-4, X linked (also known as NLGN4X, NLGN4, Neuroligin-4, and Neuroligin 4X) is a postsynaptic cell-adhesion molecule that interacts with presynaptic cell-surface neurexins. These interactions bridge together the synaptic cleft and have an essential role in synaptic transmissions between neurons. There are 5 neuroligin genes in humans: NLGN1, NLGN2, NLGN3, NLGN4X, and NLGN4Y. Mutations in NLGN3 and NLGN4X are linked to Autism Spectrum Disorders (ASD). The expression of neuroligin-4, X-linked has been reported on human pluripotent stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC22376; NLGN
Gene Aliases: NL-4; NL4; Nlgn4; Nlgn4x
Entrez Gene ID: (Mouse) 100113365
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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