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Invitrogen
KCNK3 Monoclonal Antibody (N374/48), FITC
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FIGURE: 1 / 3
KCNK3 Antibody (MA5-45702) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with TASK1 Potassium Channel monoclonal antibody (Product # MA5-45702) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Re... View More
Product Details
MA5-45702
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
N374/48
Immunogen
Fusion protein amino acids 251-411 (cytoplasmic C-terminus) of rat Acid-sensitive potassium channel protein TASK or TASK1.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Mouse: 96% identity (156/161 amino acids identical). Human: 76% identity (163/161 amino acids identical). <30% identity with TASK3.
1 µg/mL of MA5-45702 was sufficient for detection of TASK1 Potassium Channel in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 50kDa. Does not cross-react with TASK3.
This antibody was formerly sold as clone S374-48.
Target Information
KCNK3 encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The gene product is an outwardly rectifying channel that is sensitive to changes in extracellular pH and is inhibited by extracellular acidification. Also referred to as an acid-sensitive potassium channel, it is activated by the anesthetics halothane and isoflurane. Although three transcripts are detected in northern blots, there is currently no sequence available to confirm transcript variants for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acid-sensitive potassium channel protein TASK; Acid-sensitive potassium channel protein TASK-1; cardiac potassium channel; Cardiac two pore background K(+) channel; cTBAK 1; cTBAK-1; KCNK9; Potassium channel subfamily K member 3; potassium channel, subfamily K, member 3; potassium channel, two pore domain subfamily K, member 3; potassium inwardly-rectifying channel, subfamily K, member 3; TASK 1; TWIK-related acid-sensitive K(+) channel 1; TWIK-related acid-sensitive K+ 1; TWIK-related acid-sensitive K+ channel; two P domain potassium channel; two P domain potassium channel; cardiac potassium channel; KCNK3; Two pore K(+) channel KT3.1; Two pore potassium channel KT3.1
Gene Aliases: Ctbak; cTBAK-1; K2p3.1; KCNK3; OAT1; PPH4; rTASK; TASK; TASK-1; TASK1; TBAK1
UniProt ID: (Human) O14649, (Rat) O54912, (Mouse) O35111
Entrez Gene ID: (Human) 3777, (Rat) 29553, (Mouse) 16527
ion channel activity
open rectifier potassium channel activity
potassium channel activity
sodium channel activity
protein binding
outward rectifier potassium channel activity
potassium ion leak channel activity
S100 protein binding
metal ion binding
protein heterodimerization activity
protein C-terminus binding
protein homodimerization activity
voltage-gated ion channel activity
ion channel
transporter
detection of hypoxic conditions in blood by carotid body chemoreceptor signaling
ion transport
potassium ion transport
sodium ion transport
chemical synaptic transmission
response to xenobiotic stimulus
ion transmembrane transport
sodium ion transmembrane transport
negative regulation of cytosolic calcium ion concentration
regulation of resting membrane potential
cellular response to zinc ion
cellular response to hypoxia
cellular response to acidic pH
potassium ion transmembrane transport
cochlea development
regulation of action potential firing rate
brain development
regulation of ion transmembrane transport
response to drug
transport
stabilization of membrane potential
mitophagy in response to mitochondrial depolarization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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