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Invitrogen
GABRA4 Monoclonal Antibody (N398A/34), PE
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FIGURE: 1 / 3
GABRA4 Antibody (MA5-45736) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with GABA-A Receptor Alpha4 monoclonal antibody (Product # MA5-45736) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Re... View More
Product Details
MA5-45736
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N398A/34
Immunogen
Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:100 dilution of MA5-45736 was sufficient for detection of GABA-A R, Alpha4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 60kDa. Does not cross-react with other GABA-A-Receptor Alpha subunits.
This antibody was formerly sold as clone S398A-34.
Target Information
Gamma-aminobutyric acid is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. This gene encodes subunit alpha-4, which is involved in the etiology of autism and eventually increases autism risk through interaction with another subunit, gamma-aminobutyric acid receptor beta-1. Alternatively spliced transcript variants encoding different isoforms have been found in this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GABA(A) receptor; GABA(A) receptor subunit alpha-4; GABA(A) receptor, alpha 4; GABA-A receptor alpha-4 subunit; GABAAR subunit alpha-4; GABAARalpha4; gamma-aminobutyric acid; gamma-aminobutyric acid (GABA) A receptor, alpha 4; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 4; gamma-aminobutyric acid A receptor alpha 4; Gamma-aminobutyric acid receptor subunit alpha-4; gamma-aminobutyric acid type A receptor alpha4 subunit; unnamed protein product
Gene Aliases: Gabra-4; GABRA4
UniProt ID: (Human) P48169, (Mouse) Q9D6F4, (Rat) P28471
Entrez Gene ID: (Human) 2557, (Mouse) 14397, (Rat) 140675
transmembrane signaling receptor activity
GABA-A receptor activity
ion channel activity
extracellular ligand-gated ion channel activity
chloride channel activity
benzodiazepine receptor activity
GABA-gated chloride ion channel activity
signaling receptor activity
transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential
ligand-gated ion channel
ion transport
chloride transport
gamma-aminobutyric acid signaling pathway
central nervous system development
ion transmembrane transport
synaptic transmission, GABAergic
regulation of postsynaptic membrane potential
chloride transmembrane transport
inhibitory synapse assembly
transport
regulation of response to drug
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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