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Invitrogen
NPAS4 Monoclonal Antibody (N408/79), PE
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FIGURE: 1 / 2
NPAS4 Antibody (MA5-45752) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with NPAS4 monoclonal antibody (Product # MA5-45752) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45752
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N408/79
Immunogen
Fusion protein amino acids 597-802 (C-terminus) of rat Npas4.
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:100 dilution of MA5-45752 was sufficient for detection of NPAS4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 90kDa.approximately
This antibody was formerly sold as clone S408-79.
Target Information
NXF is a member of the basic helix-loop-helix-PER (MIM 602260)-ARNT (MIM 126110)-SIM (MIM 600892) (bHLH-PAS) class of transcriptional regulators, which are involved in a wide range of physiologic and developmental events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLH-PAS type transcription factor NXF; class E basic helix-loop-helix protein 79; HLH-PAS transcription factor NXF; LE-PAS; limbic-enhanced PAS protein; neuronal PAS4; PAS domain-containing protein 10; PASD10 and LE-PAS; unnamed protein product
Gene Aliases: bHLHe79; LE-PAS; NXF; PASD10
UniProt ID: (Human) Q8IUM7, (Mouse) Q8BGD7, (Rat) Q8CJH6
Entrez Gene ID: (Human) 266743, (Mouse) 225872, (Rat) 266734
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
protein binding
macromolecular complex binding
protein heterodimerization activity
protein dimerization activity
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
learning
short-term memory
long-term memory
regulation of synaptic transmission, GABAergic
cellular response to stress
social behavior
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of synaptic plasticity
excitatory postsynaptic potential
inhibitory postsynaptic potential
cellular response to corticosterone stimulus
inhibitory synapse assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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