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Invitrogen
c-Fos Monoclonal Antibody (5B10), PerCP
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FIGURE: 1 / 2
c-Fos Antibody (MA5-45888) in IHC
Immunohistochemistry analysis using Brain. Samples were incubated with c-Fos monoclonal antibody (Product # MA5-45888) at 1:100 for Overnight at 4C, then 30 min at 37C, followed by Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain used was DAPI for 3 min at RT. Magnification: 10X.
Product Details
MA5-45888
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5B10
Immunogen
Synthetic peptide from the N-terminal of Human c-Fos (aa. 280-380)
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
A 1:1,000 dilution of MA5-45888 was sufficient for detection of c-fos in 10 µg of serum starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.
Target Information
FBJ murine osteosarcoma viral oncogene homolog is commonly known as Cellular protooncogene Fos (c-Fos). It belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14. c-Fos heterodimerizes with c-Jun to form the Activator Protein -1 (AP-1) transcription factor which regulates transcription of several genes that play an important role in cellular signal transduction, cell proliferation and differentiation. c-Fos has been shown to be phosphorylated by various stimuli including growth factors and insulin at atleast seven different sites. The best known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK which regulates localization of c-Fos to the nucleus and is thus important for c-Fos induced transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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