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Invitrogen

VPS35 Monoclonal Antibody (8A3), APC

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VPS35 Monoclonal Antibody (8A3), APC

Product Details

MA5-45942

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

1:100
-

Immunocytochemistry (ICC/IF)

1:200
-

Immunoprecipitation (IP)

1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8A3

Immunogen

Full length recombinant human VSP35
View immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932396

Product Specific Information

A 1:1,000 dilution of MA5-45942 was sufficient for detection of VPS35 in 10 µg of SH-SY5Y by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.|Detects approximately 92 kDa.

Target Information

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp434E1211; DKFZp434P1672; FLJ10752; FLJ13588; FLJ20388; hVPS35; Maternal embryonic message 3; Maternal-embryonic 3; MEM3; similar to Homo sapiens vacuolar sorting protein 35 (VPS35) mRNA with GenBank Accession Number AF191298; unnamed protein product; vacuolar protein sorting 35 homolog; Vacuolar protein sorting-associated protein 35; Vesicle protein sorting 35; VPS35

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Gene Aliases: AI647796; MEM3; PARK17; TCCCTA00141; VPS35

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UniProt ID: (Human) Q96QK1, (Mouse) Q9EQH3

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Entrez Gene ID: (Human) 55737, (Rat) 25479, (Mouse) 65114

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Function(s)
protein binding D1 dopamine receptor binding protein transporter activity membrane traffic protein
Process(es)
intracellular protein transport lysosome organization positive regulation of signal transduction positive regulation of gene expression negative regulation of gene expression regulation of mitochondrion organization protein transport Wnt signaling pathway regulation of macroautophagy regulation of protein stability protein destabilization endocytic recycling negative regulation of protein homooligomerization protein localization to organelle protein localization to endosome retrograde transport, endosome to Golgi regulation of protein catabolic process mitochondrial fragmentation involved in apoptotic process transcytosis positive regulation of protein catabolic process negative regulation of inflammatory response regulation of developmental process modulation of synaptic transmission voluntary musculoskeletal movement regulation of transport regulation of protein metabolic process positive regulation of dopamine receptor signaling pathway positive regulation of Wnt protein secretion regulation of synapse maturation positive regulation of mitochondrial fission positive regulation of canonical Wnt signaling pathway positive regulation of locomotion involved in locomotory behavior neurotransmitter receptor transport, endosome to postsynaptic membrane vesicle-mediated transport in synapse mitochondrion to lysosome transport regulation of postsynapse organization neurotransmitter receptor transport, endosome to plasma membrane regulation of postsynapse assembly negative regulation of late endosome to lysosome transport regulation of dendritic spine maintenance positive regulation of dopamine biosynthetic process negative regulation of cellular protein localization positive regulation of cellular protein localization positive regulation of protein localization to cell periphery negative regulation of lysosomal protein catabolic process regulation of presynapse assembly regulation of terminal button organization retrograde transport, endosome to plasma membrane vacuolar protein processing transport
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