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Invitrogen
CD97 Recombinant Rabbit Monoclonal Antibody (024), PE
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FIGURE: 1 / 1
CD97 Antibody (MA5-46732) in Flow
Flow cytometry analysis of CD97 in stimulated human peripheral blood mononuclear cells (PBMC) using CD97, PE Recombinant Monoclonal (Product # MA5-46732). Human PBMC were stimulated with immobilized anti-human CD3 antibody (1 μg/mL for coating culture wells), in the presence of recombinant human IL-2 (200 IU/mL final concentration) for 7 days.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-46732
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
024
Immunogen
Recombinant protein ecoding Human CD97 (Met1-Gln 398).
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
100 µg/mL
Purification
Protein A
Storage buffer
PBS with 0.5% BSA
Contains
0.1% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD97 (Met1-Gln398) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Target Information
CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adhesion G protein-coupled receptor E5; CD97; Leukocyte antigen CD97
Gene Aliases: ADGRE5; CD97
Entrez Gene ID: (Human) 976
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