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Invitrogen
EB1/EB2/EB3 Monoclonal Antibody (KT65)
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FIGURE: 1 / 1
EB1/EB2/EB3 Antibody (MA5-47713) in WB
Western blot of EB1/EB2/EB3 in mouse brain (lanes 1-5). The blot was probed with rat EB1 monoclonal antibody (Product # MA5-47663) (lane 1), EB1/EB2/EB3 monoclonal antibody (Product # MA5-47713) (lane 2), MAPRE2 monoclonal antibody (Product # MA5-47664) (lane 3), MAPRE3 monoclonal antibody (Product # MA5-47714) (lane 4), and anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.
Product Details
MA5-47713
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
KT65
Immunogen
Clone KT65 was generated from a GST fusion protein containing amino acids from the C-terminal region mouse EB1. This sequence is highly conserved in human and rat EB1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody detects a 30 kDa* protein corresponding to the molecular mass of EB1 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects EB1 by immunocytochemistry in HeLa and A431 cells. The antibody may weakly detect endogenous EB2 and EB3 in some cells and tissues.
Target Information
The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and interactions by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions with organelles and macromolecular complexes. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. The CLIP-associated protein (CLASP) family includes CLASP1 and CLASP2 proteins, which are expressed as long (alpha) and short (beta) isoforms. Thse +TIPs conatin an N-terminal TOG domain, multiple TOG-like domains, and a basic and serine-rich motif (SxIP). The TOG domain facilitates interaction with tubulin dimers, while the SxIP motif promotes interaciton with EB1 and MTs. A C-terminal domain is involved in interaction with CLIPs, as well as several other proteins. CLASPs are MT stabilizing fators that localize to mitotic spindles, kinetochores, and the midbody. CLASPs are important for cell division, and may regulate cell migration and neuronal growth cone motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adenomatosis polyposis coli binding protein Eb1; adenomatous polyposis coli-binding protein EB1; APC; APC binding protein; APC-binding protein EB1; APC-binding protein EB2; EB1; EB1 protein family member 3; EB2; EB3; EBF3; End-binding protein 1; End-binding protein 2; End-binding protein 3; MAPRE2; MAPRE3; Microtubule RP/EB; Microtubule-associated protein RP/EB family member 1; Microtubule-associated protein RP/EB family member 2; Microtubule-associated protein RP/EB family member 3; RP3; T-cell activation protein, EB1 family; T-cell activation protein, EB1 family, member 2; unnamed protein product
Gene Aliases: 5530600P05Rik; AI314113; AI462499; AI504412; AI790651; AW260097; BIM1p; C820009F03Rik; CSCSC2; D18Abb1e; D2Ertd459e; EB1; EB2; EB3; EBF3; EBF3-S; MAPRE1; MAPRE2; MAPRE3; RP1; RP3
UniProt ID: (Human) Q15691, (Human) Q15555, (Human) Q9UPY8, (Rat) Q66HR2, (Mouse) Q61166, (Rat) Q3B8Q0, (Mouse) Q8R001, (Mouse) Q6PER3, (Rat) Q5XIT1
Entrez Gene ID: (Human) 22919, (Human) 10982, (Human) 22924, (Rat) 114764, (Mouse) 13589, (Rat) 679221, (Mouse) 212307, (Mouse) 100732, (Rat) 298848
establishment of mitotic spindle orientation
microtubule bundle formation
protein localization
cell migration
regulation of microtubule polymerization or depolymerization
negative regulation of microtubule polymerization
positive regulation of microtubule polymerization
protein localization to microtubule
microtubule polymerization
spindle assembly
cell division
attachment of mitotic spindle microtubules to kinetochore
protein localization to centrosome
protein localization to mitotic spindle
protein localization to astral microtubule
non-motile cilium assembly
positive regulation of ARF protein signal transduction
positive regulation of keratinocyte migration
positive regulation of focal adhesion disassembly
regulation of microtubule polymerization
positive regulation of transcription, DNA-templated
behavioral response to cocaine
positive regulation of dendritic spine morphogenesis
mitotic nuclear division
positive regulation of microtubule plus-end binding
cell cycle
regulation of microtubule plus-end binding
positive regulation of cyclin-dependent protein serine/threonine kinase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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