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Invitrogen
Ki-67 Recombinant Rabbit Monoclonal Antibody (SP6)
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FIGURE: 1 / 33
Ki-67 Antibody (MA5-14520) in ICC/IF
Immunofluorescence analysis of Ki-67 was performed using 70% confluent log phase HeLa cells serum starved (SS) for 36 hours followed by serum release (SR) for 6 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Ki-67 Monoclonal Antibody (SP6) (Product # MA5-14520) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) H... View More
Product Details
MA5-14520
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bovine,
Cat,
Chicken,
Dog,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Rhesus monkey,
Sheep
Host/Isotype
Rabbit
/ IgG
Expression System
proprietary
Class
Recombinant Monoclonal
Type
Antibody
Clone
SP6
Immunogen
Synthetic peptide within Human Ki67 aa 1200-1300
Conjugate
Form
Liquid
Concentration
0.033 mg/mL
Amount
16.5 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Staining of formalin-fixed tissues requires boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Recommended positive controls:
IHC (P) - Human tonsil and testis tissue, common marmoset spleen tissue, rat esophagus, small intestine and liver tissue, mouse embryonic skin tissue
IHC (F) - Rat lymph node tissue, transgenic mouse spinal cord tissue
ICC/IF - HAP1 cells, human cardiac stem cells, HEp-2 cells, rat cardiomyocytes
Flow - HAP1 cells
Target Information
Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen Ki67; Molecular Immunology Borstel antibody 1; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; unnamed protein product
Gene Aliases: D630048A14Rik; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105
UniProt ID: (Human) P46013, (Mouse) E9PVX6
Entrez Gene ID: (Human) 4288, (Rat) 291234, (Mouse) 17345
DNA metabolic process
meiotic nuclear division
cell proliferation
response to organic cyclic compound
hyaluronan metabolic process
organ regeneration
cellular response to heat
chromosome segregation
regulation of mitotic nuclear division
regulation of chromatin organization
hepatocyte proliferation
cholangiocyte proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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