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Invitrogen
CD22 Monoclonal Antibody (S-HCL-1)
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FIGURE: 1 / 3
CD22 Antibody (MA562247) in WB
Western blot analysis of CD22 in whole cell extracts. Samples were incubated with CD22 monoclonal antibody (Product # MA562247) using a dilution of 2 µg/mL followed by IRDye 800CW Goat-anti-Mouse IgG (green). Non-reducing conditions (nitrocellulose membrane blot). The reactivity of S-HCL-1 antibody was assessed by comparing binding signals in a panel of three human B cell and one T cell line. Whole cell extracts (RIPA lysis buffer) of Raji, Ramos, Daudi, and Jurkat cell lines, mixed and heated (100°C, 5 min) without reducing agent in SDS-l... View More
Product Details
MA562247
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
S-HCL-1
Immunogen
Whole hairy cell leukemia cells and membrane preparation.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 8
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
CD22, also known as BL-CAM, is a type I transmembrane glycoprotein composed of two polypeptide chains, CD22alpha and CD22beta, with molecular weights of 130 and 140 kDa, respectively. These chains are produced by alternative splicing of the CD22 gene. CD22 is prominently expressed on mature B cells and B cell lymphomas, including hairy cell leukemia, diffuse large B-cell lymphoma, and nodular lymphocyte predominance Hodgkin's lymphoma, but is negative in classical Hodgkin's lymphoma. The extracellular portion of CD22 contains seven Ig-like domains that preferentially bind alpha2,6-linked sialic acid moieties found on epithelial, endothelial, B, and T cells. This binding can be masked by cis interactions with sialic acids on the same cell surface. CD22 expression is limited to late stages of B-cell differentiation, making it useful for phenotyping mature leukemias. Intracellularly, CD22 features six tyrosine residues within immunotyrosine-based inhibitory motifs (ITIM) and activation-like motifs. These residues are phosphorylated upon B-cell receptor engagement, allowing CD22 to regulate B-cell receptor signaling. CD22 participates in positive regulation through interactions with Src family tyrosine kinases and acts as an inhibitory receptor by recruiting cytoplasmic phosphatases via SH2 domains, which block signal transduction through dephosphorylation of signaling molecules. CD22's role in both positive and negative regulation of B-cell signaling, along with its specific expression pattern, makes it a valuable marker for antibody customers interested in B-cell-related research and diagnostics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell receptor CD22; B-lymphocyte cell adhesion molecule; B-lymphocyte cell adhesion molecule (BL-CAM); BL-CAM; CD22; CD22 antigen; FLJ22814; Lectin 2; Leu-14; MGC130020; Sialic acid-binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2 (Siglec-2); Siglec-2; T-cell surface antigen Leu-14; unnamed protein product
Gene Aliases: CD22; SIGLEC-2; SIGLEC2
UniProt ID: (Human) P20273
Entrez Gene ID: (Human) 933
negative regulation of immunoglobulin production
negative regulation of immune system process
cell adhesion
negative regulation of signal transduction
regulation of endocytosis
regulation of B cell proliferation
B cell activation
regulation of immune response
negative regulation of calcium-mediated signaling
negative regulation of B cell receptor signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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