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Invitrogen
CRTH2 Monoclonal Antibody (BM16), Alexa Fluor™ 488
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FIGURE: 1 / 3
CRTH2 Antibody (MA562279) in Flow
Flow cytometry (multicolor surface staining) of CRTH2 in human lymphocytes. Samples were stained with CRTH2 monoclonal antibody (Product # MA562279) using a dilution of 4 µL reagent/100 µL of peripheral whole blood with anti-human CD3 (UCHT1) APC antibody at 10 µL reagent/100 µL of peripheral whole blood.
Product Details
MA562279
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
BM16
Immunogen
Human CD294 transfected rat cell line TART/B19-12.10.
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
CRTH2 (GPR44) is a chemoattractant receptor-homologous molecule expressed on Th2 cells that is an identified receptor for the prostaglandin D2 (PGD2) and prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo. CRTH2 is also a novel member of the G protein-coupled leukocyte chemoattractant receptor family, which is selectively expressed in Th2 but not Th1 lineage cells. CRTH2 has been reported to be expressed in the central nervous system, peripheral tissues, and in blood cells, particularly activated T helper-2 cells. Two transcripts have been isolated: a 3.5 kb transcript expressed primarily in the thalamus, frontal cortex, pons and hippocampus; and a 3.4 kb transcript expressed in fetal liver, leukocytes and thymus. CRTH2 is selectively expressed in vivo in an activated state of Th2 cells including allergen-responsive Th2 cells, suggesting its pivotal roles in ongoing Th2-type immune reactions. At least two different isoforms of CRTH2 are known to exist. Single nucleotide polymorphisms in the 3' UTR of the CRTH2 gene have been associated with asthma susceptibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD294; chemoattractant receptor homologous molecule expressed on T helper type 2 cells; Chemoattractant receptor-homologous molecule expressed on Th2 cells; CRTH2/GPR44; G-protein coupled receptor; DL1R; G-protein coupled receptor 44; MGC130436; Prostaglandin D2 receptor 2; putative G-protein coupled receptor 44; unnamed protein product
Gene Aliases: CD294; CRTH2; DL1R; DP2; GPR44; PTGDR2
UniProt ID: (Human) Q9Y5Y4
Entrez Gene ID: (Human) 11251
chemotaxis
immune response
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
neuropeptide signaling pathway
calcium-mediated signaling
positive regulation of G-protein coupled receptor protein signaling pathway
cellular response to prostaglandin D stimulus
negative regulation of male germ cell proliferation
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