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Perforin Monoclonal Antibody (dG9), APC
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FIGURE: 1 / 3
Perforin Antibody (MA562394) in Flow
Flow cytometry (intracellular staining) of Perforin in human peripheral whole blood. Samples were stained with Perforin monoclonal antibody (Product # MA562394) using a dilution of 10 µL reagent/100 µL of peripheral whole blood. Separation of human Perforin positive CD56 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).
Product Details
MA562394
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
dG9
Immunogen
Purified granules from human YT lymphoma cell line.
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytolysin; Lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1 (pore forming protein); Perforin-1; Perforin1; PFP; PGFL; PIGF; PIGF-2; PLGF; pore forming protein; PRF1 (pore forming protein 1); RP11-710A11.3; unnamed protein product
Gene Aliases: HPLH2; P1; PFP; PRF1
UniProt ID: (Human) P14222
Entrez Gene ID: (Human) 5551, (Bovine) 369025
immunological synapse formation
plasma membrane repair
leukocyte mediated cytotoxicity
T cell mediated cytotoxicity
defense response to tumor cell
immune response to tumor cell
positive regulation of immune response to tumor cell
apoptotic process
cellular defense response
protein secretion
protein import
killing of cells of other organism
ceramide biosynthetic process
protein homooligomerization
protein maturation
defense response to virus
positive regulation of killing of cells of other organism
protein transmembrane transport
granzyme-mediated programmed cell death signaling pathway
pyroptotic cell death
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