Hamburger Menu Button
Thermo Fisher Scientific Logo
登入
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Tubes
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Clinical Genomics
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO 和 CRO 服務
    • CDMO 服務
    • 臨床試驗 CRO 服務
    • Custom Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • Register for an Account
    • How to Order
    • Instrument Support
    • Technical Support Centers
    • Learning Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Who We Serve
    • Biotech
    • Biopharma
    • CDMO
    • Lab Diagnostics
    • Industrial and Applied Sciences
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search

Search All
Search button
          • Order Status
          • 快速訂購
          • 登入
            登入
            Don't have an account ? Create Account
            • 帳戶
            • 訂單
            • 訂製產品與專案
          • Primary Antibodies ›
          • LYN/LCK/HCK/BLK Antibodies

          Invitrogen

          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Recombinant Monoclonal Antibody (PSH09-43)

          Advanced Verification

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support

          Cite Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Recombinant Monoclonal Antibody (PSH09-43)

          Additional Information:
          {{banner.description}}
          {{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
          • Antibody Testing Data (5)
          • Advanced Verification (3)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody (MA562902) in ICC/IF

          Immunofluorescence analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) using Ramos cells serum starved for 16 hours then treated with 12μg/mL human IgM (diluted in serum free medium) for 2 minutes. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Western Blot (WB)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Western Blot (WB)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Western Blot (WB)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Western Blot (WB)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Recombinant Monoclonal Antibody (PSH09-43)

          Product Details

          MA562902

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000
          -

          Immunocytochemistry (ICC/IF)

          1:100
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Artificial Control / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          PSH09-43

          Immunogen

          Synthetic phospho-peptide corresponding to residues surrounding Tyr394 of human LCK.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4, with 0.1% BSA, 40% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3719757

          Product Specific Information

          Positive Control: COLO205 cell lysate, TT cell lysate, Ramos serum starved for 16 hours cell lysate, Ramos serum starved for 16 hours add 12 µg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 50mM sodium orthovanadate for 5 minutes cell lysate, PC-12 cell lysate, PC-12 treated with 1mM sodium orthovanadate for 30 minutes cell lysate, Ramos cells serum starved for 16 hours add 12 µg/mL human IgM (diluted in serum free medium) for 2 minutes.

          Tissue Specificity: Tissue enhanced (bone marrow, lymphoid tissue).

          Subcellular Location: Cell membrane, Cytoplasm, cytosol.

          Sequence Similarities: 100% Mouse/Rat.

          Predicted band size: 58 kDa

          Target Information

          The LYN, LCK, HCK, and BLK genes encode various members of the Src family of non-receptor tyrosine kinases, each playing crucial roles in cellular signaling pathways that regulate immune function, cell proliferation, and differentiation. LYN is involved in B-cell receptor signaling, influencing cell proliferation, differentiation, apoptosis, migration, metabolism, and roles in inflammation and cancer, particularly within the immune system and oncogenic pathways. LCK is primarily expressed in T-cells and is crucial for T-cell development, function, and signaling, impacting cytokine production and immune responses. HCK, primarily found in hematopoietic cells, influences myeloid cell function and is implicated in oncogenic signaling pathways, potentially contributing to cancer progression via altered immune responses. BLK participates mainly in B-cell receptor signaling, impacting B-cell development and activation, with implications for immune cell signaling and potential roles in autoimmune diseases. Each of these kinases is a potential therapeutic target, given their roles in various pathophysiological conditions and their impact on immune cell signaling and cancer.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

          Have you cited this product in a publication?
          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: B cell/myeloid kinase; B lymphocyte kinase; B lymphoid tyrosine kinase; B-cell/myeloid kinase; BLK nonreceptor tyrosine kinase; BMK; Hematopoietic cell kinase; Hemopoietic cell kinase; hemopoietic cell tyrosine kinase; Lck/Yes-related novel protein tyrosine kinase; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte protein tyrosine kinase; lymphocyte-specific protein tyrosine kinase; lyn protein non-receptor kinase; lyn tyrosine kinase; p53Lyn; p55-Blk; p56(LSTRA) protein-tyrosine kinase; p56-HCK; p56-HCK/p59-HCK; p56-LCK; p56Hck; p56Lyn; p59-HCK/p60-HCK; p59Hck; p61Hck; protein tyrosine kinase; Protein YT16; protein-tyrosine kinase; Proto-oncogene Lck; Proto-oncogene tyrosine-protein kinase LCK; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; Tyrosine-protein kinase Blk; Tyrosine-protein kinase HCK; Tyrosine-protein kinase Lck; Tyrosine-protein kinase Lyn; unnamed protein product; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v-yes-1) oncogene homolog

          View more View less

          Gene Aliases: AA407514; AI849071; AIPCV; BLK; Bmk; HCK; Hck-2; Hck-3; Hctk; IMD22; JTK8; JTK9; LCK; Lck1; Lcktkr; LSK; Lsk-t; Lskt; LYN; MODY11; p53Lyn; p56; p56Hck; p56Lck; p56Lyn; p59Hck; p61Hck; pp58lck; SAIDV; YT16

          View more View less

          UniProt ID: (Human) P51451, (Mouse) P16277, (Human) P08631, (Rat) P50545, (Mouse) P08103, (Human) P06239, (Human) P07948, (Rat) Q01621, (Mouse) P06240, (Mouse) P25911, (Rat) Q07014

          View more View less

          Entrez Gene ID: (Human) 640, (Mouse) 12143, (Rat) 364403, (Human) 3055, (Rat) 25734, (Mouse) 15162, (Human) 3932, (Human) 4067, (Rat) 313050, (Mouse) 16818, (Mouse) 17096, (Rat) 81515

          View more View less

          Function(s)
          nucleotide binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups molecular_function phosphotyrosine binding lipid binding protein serine/threonine phosphatase activity phospholipase activator activity protein kinase binding protein phosphatase binding SH2 domain binding T cell receptor binding CD4 receptor binding CD8 receptor binding identical protein binding phospholipase binding phosphatidylinositol 3-kinase binding ATPase binding protein antigen binding platelet-derived growth factor receptor binding integrin binding SH3 domain binding enzyme binding receptor activator activity ubiquitin protein ligase binding gamma-tubulin binding glycosphingolipid binding ion channel binding macromolecular complex binding ephrin receptor binding phosphoprotein binding scaffold protein binding phosphorylation-dependent protein binding phosphatidylinositol 3-kinase activator activity glycoprotein binding antigen binding protein C-terminus binding protein complex binding erythropoietin receptor binding
          Process(es)
          immune system process leukocyte migration involved in immune response innate immune response-activating signal transduction negative regulation of inflammatory response to antigenic stimulus protein phosphorylation exocytosis phagocytosis response to stress inflammatory response cell adhesion transmembrane receptor protein tyrosine kinase signaling pathway integrin-mediated signaling pathway mesoderm development positive regulation of cell proliferation regulation of cell shape peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway cell differentiation positive regulation of actin filament polymerization lipopolysaccharide-mediated signaling pathway regulation of actin cytoskeleton organization intracellular signal transduction Fc-gamma receptor signaling pathway involved in phagocytosis negative regulation of apoptotic process leukocyte degranulation innate immune response respiratory burst after phagocytosis protein autophosphorylation regulation of inflammatory response regulation of phagocytosis defense response to Gram-positive bacterium leukocyte migration regulation of sequence-specific DNA binding transcription factor activity interferon-gamma-mediated signaling pathway cellular response to lipid regulation of podosome assembly phosphorylation T cell differentiation positive regulation of insulin secretion peptidyl-tyrosine autophosphorylation regulation of cell proliferation B cell receptor signaling pathway cellular response to peptide hormone stimulus B cell homeostasis regulation of cytokine production negative regulation of protein phosphorylation Fc receptor mediated stimulatory signaling pathway tolerance induction to self antigen histamine secretion by mast cell platelet degranulation negative regulation of myeloid leukocyte differentiation immune response-regulating cell surface receptor signaling pathway Fc receptor mediated inhibitory signaling pathway regulation of B cell apoptotic process cellular response to DNA damage stimulus signal transduction negative regulation of cell proliferation response to hormone positive regulation of neuron projection development hemopoiesis erythrocyte differentiation positive regulation of cell migration negative regulation of B cell proliferation neuron projection development regulation of mast cell activation regulation of cell adhesion mediated by integrin negative regulation of toll-like receptor 2 signaling pathway negative regulation of toll-like receptor 4 signaling pathway positive regulation of phosphorylation positive regulation of tyrosine phosphorylation of STAT protein hemoglobin biosynthetic process regulation of mast cell degranulation negative regulation of MAP kinase activity positive regulation of phosphatidylinositol 3-kinase activity regulation of erythrocyte differentiation cytokine secretion regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation regulation of B cell receptor signaling pathway positive regulation of B cell receptor signaling pathway positive regulation of cellular component movement regulation of release of sequestered calcium ion into cytosol positive regulation of glial cell proliferation positive regulation of Fc receptor mediated stimulatory signaling pathway positive regulation of stress-activated protein kinase signaling cascade regulation of ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of oligodendrocyte progenitor proliferation negative regulation of mast cell proliferation positive regulation of mast cell proliferation cellular response to retinoic acid regulation of monocyte chemotaxis regulation of platelet aggregation dendritic cell differentiation negative regulation of intracellular signal transduction positive regulation of dendritic cell apoptotic process cytoskeleton organization positive regulation of actin cytoskeleton reorganization cellular zinc ion homeostasis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cell surface receptor signaling pathway aging response to mechanical stimulus response to metal ion response to zinc ion positive regulation of gene expression dephosphorylation response to drug positive regulation of tyrosine phosphorylation of Stat5 protein response to hydrogen peroxide regulation of apoptotic process positive regulation of gamma-delta T cell differentiation regulation of T cell receptor signaling pathway positive regulation of T cell receptor signaling pathway positive regulation of T cell activation release of sequestered calcium ion into cytosol positive regulation of uterine smooth muscle contraction positive regulation of intrinsic apoptotic signaling pathway DNA damage checkpoint cell morphogenesis regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway hematopoietic progenitor cell differentiation adaptive immune response autophagy response to sterol depletion response to xenobiotic stimulus response to toxic substance response to carbohydrate oligodendrocyte development fatty acid transport eosinophil differentiation T cell costimulation response to insulin toll-like receptor 4 signaling pathway cellular response to heat interleukin-5-mediated signaling pathway Fc-epsilon receptor signaling pathway C-X-C chemokine receptor CXCR4 signaling pathway B cell proliferation response to amino acid positive regulation of MAPK cascade response to peptide hormone ephrin receptor signaling pathway response to axon injury negative regulation of immune response negative regulation of B cell receptor signaling pathway JAK-STAT cascade involved in growth hormone signaling pathway neuroinflammatory response positive regulation of amyloid precursor protein catabolic process positive regulation of protein localization to plasma membrane chemotaxis response to organic cyclic compound cell migration cellular response to extracellular stimulus immune response-activating cell surface receptor signaling pathway platelet activation positive regulation of heterotypic cell-cell adhesion Fc-gamma receptor signaling pathway T cell activation gamma-delta T cell differentiation regulation of regulatory T cell differentiation T cell receptor signaling pathway positive regulation of multicellular organismal process regulation of lymphocyte activation CD27 signaling pathway positive regulation of leukocyte cell-cell adhesion biological_process
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us About Us
          • Careers Careers
          • Investors Investors
          • News News
          • Social Responsibility Social Responsibility
          • Trademarks
          • Policies and Notices
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Policy
          • Price & Freight Policy
          © 2006-2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          Hong Kong flag icon
          Hong Kong

          Your items have has been added!


          Host server : magellan-search-green-b49b87d85-9fm2r:80/100.66.79.29:80.
          git-commit: 5b8c860b7cdb41e9cfe07630520f6b51e109d38e
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.47.0-Offline