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Invitrogen
MAD1 Polyclonal Antibody
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FIGURE: 1 / 9
MAD1 Antibody (PA5-79617) in ICC/IF
Immunocytochemistry analysis of MAD1 using anti-MAD1 antibody (Product # PA5-79617) . MAD1 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-MAD1 antibody (Product # PA5-79617) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More
Product Details
PA5-79617
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human MAD1 recombinant protein (Position: L362-A632).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human Raji whole cell, human Jurkat whole cell, human HepG2 whole cellhuman HL-60 whole cellhuman THP-1 whole cellhuman Caco-2 whole cell, rat brain tissue, rat kidney tissue, rat lung tissue, rat liver tissue, mouse brain tissue, mouse kidney tissue, mouse lung tissue, mouse liver tissue, mouse NIH3T3 whole cell. IHC: mouse intestine tissue, rat intestine tissue, human intestinal cancer tissue. ICC/IF: U20S cell, Hela cell. Flow: A431 cell.
Target Information
MAX dimerization protein belongs to a subfamily of MAX-interacting proteins. This protein competes with MYC for binding to MAX to form a sequence-specific DNA-binding complex, acts as a transcriptional repressor (while MYC appears to function as an activator) and is a candidate tumor suppressor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hMAD1; HsMAD1; MAD1 mitotic arrest deficient-like 1; MAD1-like protein 1; mitotic arrest deficient 1-like 1; Mitotic arrest deficient 1-like protein 1; Mitotic checkpoint MAD1 protein homolog; Mitotic spindle assembly checkpoint protein MAD1; mitotic-arrest deficient 1, yeast, homolog-like 1; MXD1; Tax-binding protein 181; tumor protein p53 inducible protein 9
Gene Aliases: AI173502; AW550425; MAD1; MAD1L1; PIG9; TP53I9; TXBP181
UniProt ID: (Human) Q9Y6D9, (Mouse) Q9WTX8
Entrez Gene ID: (Human) 8379, (Rat) 680006, (Mouse) 17120
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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