Proteintech

MAD2L1 Polyclonal Antibody

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MAD2L1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA ) fixed HEK-293 cells using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MAD2L1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 40x lens).

MAD2L1 Antibody in Western Blot (WB) — Rat liver tissue were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

MAD2L1 Antibody in Immunoprecipitation (IP) — IP result of anti-MAD2L1 (IP:10337-1-AP, 3ug; Detection:10337-1-AP 1:500) with HEK-293 cells lysate 1800ug.

Product Details

10337-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 4 publications 4 publications
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MAD2L1 Fusion Protein Ag0307 (16-185 aa encoded by BC000356) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.21 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: SAEIVAEFF SFGINSILYQ RGIYPSETFT RVQKYGLTLL VTTDLELIKY LNNVVEQLKD WLYKCSVQKL VVVISNIESG EVLERWQFDI ECDKTAKDDS APREKSQKAI QDEIRSVIRQ ITATVTFLPL LEVSCSFDLL IYTDKDLVVP EKWEESGPQF ITNSEEVRLR S (16-185 aa encoded by BC000356)

Target Information

Tight junction proteins (TJPs) belong to a family of membrane-associated guanylate kinase (MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. TJPs bind to the cytoplasmic C termini of junctional transmembrane proteins and link them to the actin cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nucleoporin35 is a novel microtubule associated protein functioning in oocyte meiotic spindle architecture.

Experimental cell research

Chen F,Jiao XF,Zhang JY,Wu D,Ding ZM,Wang YS,Miao YL,Huo LJ

Mon Oct 15 00:00:00 EDT 2018

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

Oncogene

Zhu ZC,Liu JW,Li K,Zheng J,Xiong ZQ

Tue May 01 00:00:00 EDT 2018

MPHOSPH1: a potential therapeutic target for hepatocellular carcinoma.

Cancer research

Liu X,Zhou Y,Liu X,Peng A,Gong H,Huang L,Ji K,Petersen RB,Zheng L,Huang K

Sat Nov 15 00:00:00 EST 2014

Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex.

Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine

Wu J,Chen W,Yin Y,Zheng Z,Zou G

Sun Jun 01 00:00:00 EDT 2014

Expression of the mitotic-arrest deficiency 2 is associated with chemotherapy resistance in ovarian serous adenocarcinoma.

Oncology reports

Nakano Y,Sumi T,Teramae M,Morishita M,Fukuda T,Terada H,Yoshida H,Matsumoto Y,Yasui T,Ishiko O

Mon Oct 01 00:00:00 EDT 2012

Mitotic arrest deficiency 2 induces carcinogenesis in mucinous ovarian tumors.

Oncology letters

Nakano Y,Sumi T,Morishita M,Fukuda T,Nobeyama H,Yoshida H,Matsumoto Y,Yasui T,Ishiko O

Wed Feb 01 00:00:00 EST 2012

Expression of mitotic-arrest deficiency 2 predicts the efficacy of neoadjuvant chemotherapy for locally advanced uterine cervical cancer.

Experimental and therapeutic medicine

Morishita M,Sumi T,Nakano Y,Teramae M,Fukuda T,Nobeyama H,Yoshida H,Matsumoto Y,Yasui T,Ishiko O

Wed Feb 01 00:00:00 EST 2012

Mitotic arrest defective protein 2 expression abnormality and its clinicopathologic significance in human osteosarcoma.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Yu L,Guo WC,Zhao SH,Tang J,Chen JL

Mon Mar 01 00:00:00 EST 2010

Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis.

Cancer research

He F,Mo L,Zheng XY,Hu C,Lepor H,Lee EY,Sun TT,Wu XR

Tue Dec 15 00:00:00 EST 2009

Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis.

Cancer research

He F,Mo L,Zheng XY,Hu C,Lepor H,Lee EY,Sun TT,Wu XR

Tue Dec 15 00:00:00 EST 2009

Bioinformatics

Protein Aliases: HsMAD2; MAD2 (mitotic arrest deficient, homolog)-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2-like 1; MAD2-like protein 1; MadL1; Mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; Mitotic spindle assembly checkpoint protein MAD2A

Gene Aliases: AA673185; HSMAD2; MAD2; Mad2a; MAD2L1

UniProt ID: (Human) Q13257, (Mouse) Q9Z1B5

Entrez Gene ID: (Human) 4085, (Rat) 297176, (Mouse) 56150

Function(s)