Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
MAFF Antibody (PA5-109931) in WB
Western blot analysis of MAFF in extracts of HT-29 cells. Samples were incubated with MAFF Polyclonal antibody (Product # PA5-109931) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-109931
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 75-164 of human MAFF (NP_036455.1).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.72 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855342
Product Specific Information
Immunogen sequence: QKEELQKQKS ELEREVDKLA RENAAMRLEL DALRGKCEAL QGFARSVAAA RGPATLVAPA SVITIVKSTP GSGSGPAHGP DPAHGPASCS
Target Information
The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Transcription factor MafF; U-Maf; v-maf avian musculoaponeurotic fibrosarcoma oncogene family protein F; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F; V-maf musculoaponeurotic fibrosarcoma oncogene homolog F
Gene Aliases: hMafF; MAFF; U-MAF
UniProt ID: (Human) Q9ULX9
Entrez Gene ID: (Human) 23764
in utero embryonic development
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
parturition
blood coagulation
skeletal muscle cell differentiation
regulation of epidermal cell differentiation
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support