Immunohistochemical analysis of paraformldehyde-fixed and paraffin-embedded human breast carcinoma with anti-MAGEA4 polyclonal antibody (Product # PA5-13153) at a 1:50 dilution, followed by a biotin-conjugated secondary antibody and ABC solution and detected with DAB. Cancerous cells show nuclear staining of MAGE-A4. Magnification: 40X.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human MAGEA4|
|Purification||Ammonium sulfate precipitation, Size-exclusion - Dialysis|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MAGEA4 is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in somehereditary disorders, such as dyskeratosis congenita.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.