Western blot analysis of MAL was performed by loading 50ug of mouse mpkCCD and Xenopus 2f3 whole cell lysates per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% nonfat dry milk in TBS for 1 hour at room temperature. The membrane was probed with a MAL polyclonal antibody (Product # PA1-085) at a dilution of 1:500 overnight at 4C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody for 1 hour. Detection was performed using SuperSignal West Dura (Product # 34075). Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse, Rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptides corresponding to residues C(114)F D G F T Y (R/K) H Y H E N I(126) of human MAL.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-085 detects MAL from rat, mouse, xenopus and human samples.
PA1-085 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~ 17 kDa protein corresponding to MAL from rat kidney extracts.
The PA1-085 immunogen is a synthetic peptides corresponding to residues C(114)F D G F T Y (R/K) H Y H E N I(126) of human MAL. This peptide (Cat. # PEP-249) can be used in neutralization and control experiments.
MAL, a nonglycosylated integral membrane proteolipid, has been identified as a major internal component of glycolipid-enriched membrane (GEM) microdomains. MAL is the first integral membrane proteolipid component to have been shown as a necessary element in the cellular apical sorting apparatus for newly synthesized proteins. In its steady state this highly hydrophobic proteolipid, resides predominantly in the apical zone of polarized epithelial cells. It has also been shown in itinerant protein cycling between the plasma membrane and endosomes and in other cells. This 17-kDa proteolipid contains four putative transmembrane domains. These domains along with its protein trafficking properties are defined by the specific signals delegated by its specific amino acid sequence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.