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Invitrogen
MAL Polyclonal Antibody
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FIGURE: 1 / 1
MAL Antibody (PA1-085) in WB
Western blot analysis of MAL was performed by loading 50 µg of mouse mpkCCD and Xenopus 2f3 whole cell lysates per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% nonfat dry milk in TBS for 1 hour at room temperature. The membrane was probed with a MAL polyclonal antibody (Product # PA1-085) at a dilution of 1:500 overnight at 4C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody for 1 hour. Detection was performed using SuperSignal West Dura (Product # 34075). Data ... View More
Product Details
PA1-085
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Xenopus
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptides corresponding to residues C(114)F D G F T Y (R/K) H Y H E N I(126) of human MAL.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-085 detects MAL from rat, mouse, xenopus and human samples.
PA1-085 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~ 17 kDa protein corresponding to MAL from rat kidney extracts.
The PA1-085 immunogen is a synthetic peptides corresponding to residues C(114)F D G F T Y (R/K) H Y H E N I(126) of human MAL. This peptide (Cat. # PEP-249) can be used in neutralization and control experiments.
Target Information
MAL, a nonglycosylated integral membrane proteolipid, has been identified as a major internal component of glycolipid-enriched membrane (GEM) microdomains. MAL is the first integral membrane proteolipid component to have been shown as a necessary element in the cellular apical sorting apparatus for newly synthesized proteins. In its steady state this highly hydrophobic proteolipid, resides predominantly in the apical zone of polarized epithelial cells. It has also been shown in itinerant protein cycling between the plasma membrane and endosomes and in other cells. This 17-kDa proteolipid contains four putative transmembrane domains. These domains along with its protein trafficking properties are defined by the specific signals delegated by its specific amino acid sequence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 17 kDa myelin vesicular protein; mal T-cell differentiation protein; MVP17; MyD88-adapter-like; Myelin and lymphocyte protein; myelin and lymphocyte protein, T-cell differentiation protein; NS 3; T-lymphocyte maturation-associated protein
Gene Aliases: MAL; MALGENE; Mpv17; Mvp17; Vip17; XELAEV_18026637mg
UniProt ID: (Human) P21145, (Mouse) O09198, (Rat) Q64349
Entrez Gene ID: (Human) 4118, (Mouse) 17153, (Rat) 25263, (Xenopus) 379738
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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