Invitrogen

MAN1 Polyclonal Antibody

Product Image Gallery

MAN1 Antibody in Western Blot (WB) — Western blot analysis of LEMD3 in human colon tissue lysate using LEMD3 polyclonal antibody (Product # PA5-72734) at a dilution of 1 µg/mL.

MAN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human colon tissue. The sample was probed with LEMD3 polyclonal antibody (Product # PA5-72734) with a dilution of 2.5 µg/mL.

MAN1 Antibody in Immunofluorescence (IF) — Immunocytochemistry analysis of LEMD3 in human colon cells. The samples were probed with a LEMD3 polyclonal antibody (Product # PA5-72734) at a dilution of 5 µg/mL.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

2.5 µg/mL

Immunocytochemistry (ICC)

Assay-Dependent

Immunofluorescence (IF)

5 µg/mL

ELISA (ELISA)

1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

LEMD3 antibody for an 18 amino acid synthetic peptide near the carboxy terminus of human LEMD3. The immunogen is within the last 50 amino acids of LEMD3.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2718588

Target Information

Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Inner nuclear membrane protein Man1; integral inner nuclear membrane protein; integral inner nuclear membrane protein MAN1; LEM domain-containing protein 3

Gene Aliases: AI316861; LEMD3; MAN1

UniProt ID: (Human) Q9Y2U8, (Mouse) Q9WU40

Entrez Gene ID: (Human) 23592, (Mouse) 380664

Function(s)

negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway negative regulation of activin receptor signaling pathway angiogenesis blood vessel endothelial cell migration involved in intussusceptive angiogenesis nucleus organization skeletal muscle cell differentiation regulation of cell cycle regulation of extracellular matrix organization

Process(es)

nucleotide binding DNA binding protein binding