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Invitrogen
MAN2A1 Polyclonal Antibody
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FIGURE: 1 / 5
MAN2A1 Antibody (PA5-115539) in ICC/IF
Immunocytochemistry analysis of MAN2A1 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115539) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115539
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MAN2A1(Accession Q16706), corresponding to amino acid residues Y156-D206.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Mannosidase II.
Target Information
This gene encodes a glycosyl hydrolase that localizes to the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-mannosidase 2; alpha-mannosidase II; AMan II; Golgi alpha-mannosidase II; golgi integral membrane protein 7; MAN II; mannosidase 2, alpha 1; mannosidase alpha 2; Mannosidase alpha class 2A member 1; mannosidase, alpha type II; Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Gene Aliases: AMan II; GOLIM7; MAN2A1; Mana-2; MANA2; MANII; Manno; Map-2
UniProt ID: (Human) Q16706, (Mouse) P27046, (Rat) P28494
Entrez Gene ID: (Human) 4124, (Mouse) 17158, (Rat) 25478
catalytic activity
alpha-mannosidase activity
mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity
mannosidase activity
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing N-glycosyl compounds
carbohydrate binding
metal ion binding
hydrolase activity, hydrolyzing O-glycosyl compounds
zinc ion binding
in utero embryonic development
liver development
carbohydrate metabolic process
mannose metabolic process
N-glycan processing
mitochondrion organization
vacuole organization
respiratory gaseous exchange
metabolic process
lung alveolus development
positive regulation of neurogenesis
retina morphogenesis in camera-type eye
protein glycosylation
viral protein processing
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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