Invitrogen

MAP1B Polyclonal Antibody

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MAP1B Antibody in Immunocytochemistry (ICC/IF) — MAP1B Polyclonal Antibody detects MAP1B protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAP1B protein stained by MAP1B Polyclonal Antibody (Product # PA5-78052) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500. Blue: Fluoroshield with DAPI .

MAP1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — MAP1B Polyclonal Antibody detects MAP1B protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MAP1B Polyclonal Antibody (Product # PA5-78052) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

MAP1B Antibody in Western Blot (WB) — Western Blot using MAP1B Polyclonal Antibody (Product # PA5-78052). Various whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with MAP1B Polyclonal Antibody (Product # PA5-78052) diluted at 1:2,000.

Product Details

PA5-78052

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MAP1B. The exact sequence is proprietary. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	2.49 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2736113

Product Specific Information

Positive Control: SK-N-AS, C8D30

Predicted Reactivity: Mouse (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantitative propagation of assembled human Tau from Alzheimer's disease brain in microfluidic neuronal cultures.

The Journal of biological chemistry

Katsikoudi A,Ficulle E,Cavallini A,Sharman G,Guyot A,Zagnoni M,Eastwood BJ,Hutton M,Bose S

PA5-78052 was used in Immunocytochemistry to show that a small molecule inhibitor of aggregation can block the trans-neuronal transfer of Tau aggregates, suggesting that the system can be used to evaluate mechanisms of Tau transfer and find therapeutic interventions.

Fri Sep 11 00:00:00 EDT 2020

Quantitative propagation of assembled human Tau from Alzheimer's disease brain in microfluidic neuronal cultures.

The Journal of biological chemistry

Katsikoudi A,Ficulle E,Cavallini A,Sharman G,Guyot A,Zagnoni M,Eastwood BJ,Hutton M,Bose S

Fri Sep 11 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: DKFZp686E1099; DKFZp686F1345; FLJ38954; MAP-1B; MAP1 light chain LC1; MAP1(X); MAP1.2; MAP1B heavy chain; Microtubule-associated protein 1B; Microtubule-associated protein 1B (MAP 1B) (MAP1.2) (MAP1(X)); microtubule-associated protein 5; Neuraxin; protein phosphatase 1, regulatory subunit 102

Gene Aliases: A230055D22; AI843217; FUTSCH; LC1; MAP1B; MAP5; Mtap-5; Mtap1b; Mtap5; PPP1R102

UniProt ID: (Human) P46821, (Rat) P15205, (Mouse) P14873

Entrez Gene ID: (Human) 4131, (Rat) 29456, (Mouse) 17755

Molecular Function: non-motor microtubule binding protein