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MAP3K5 Antibody (TA890021) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP3K5(RC209913, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP3K5. Positive lysates LY401794 (100 µg) and LC401794 (20 µg) can be purchased separately from OriGene.
Product Details
TA890021
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein of human MAP3K5
Conjugate
Form
Liquid
Concentration
1.18 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase that is abundantly expressed in human heart and pancreas and are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activates c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death and a catalytically inactive form of ASK1 inhibited TNF-a-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: apoptosis signal regulating kinase 1; Apoptosis signal-regulating kinase 1; ASK 1; ASK-1; EC 2.7.11.25; kinase ASK1; M3K5; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; mitogen activated protein kinase kinase kinase 5; Mitogen-activated protein kinase kinase kinase 5; RP3-325F22.4
Gene Aliases: 7420452D20Rik; ASK; ASK1; MAP3K5; MAPKKK5; MEKK5
UniProt ID: (Human) Q99683, (Mouse) O35099
Entrez Gene ID: (Human) 4217, (Mouse) 26408
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
protein kinase binding
protein phosphatase binding
protein homodimerization activity
nucleotide binding
cysteine-type endopeptidase activator activity involved in apoptotic process
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPKK activity
response to ischemia
protein phosphorylation
JNK cascade
activation of JUN kinase activity
intrinsic apoptotic signaling pathway in response to oxidative stress
viral process
response to endoplasmic reticulum stress
p38MAPK cascade
positive regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of JUN kinase activity
innate immune response
positive regulation of myoblast differentiation
positive regulation of JNK cascade
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hydrogen peroxide
apoptotic signaling pathway
programmed necrotic cell death
positive regulation of neuron death
cellular response to reactive nitrogen species
activation of MAPK activity
positive regulation of protein phosphorylation
immune system process
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of cell death
phosphorylation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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