Invitrogen

MAP4 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (17) MAP4 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

MAP4 Antibody in Immunofluorescence (IF) — Immunofluorescent staining of MAP4 in human cell line U-2 OS shows positivity in plasma membrane, cytoplasm and microtubules. Samples were probed using a MAP4 Polyclonal Antibody (Product # PA5-58273).

MAP4 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of MAP4 in human kidney, skeletal muscle, soft tissues and testis using MAP4 Polyclonal Antibody (Product # PA5-58273) (A) shows similar protein distribution across tissues to an independent MAP4 Polyclonal Antibody (B).

MAP4 Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of MAP4 demonstrates antibody specificity. Immunohistochemical analysis of MAP4 using anti-MAP4 Polyclonal Antibody (Product # PA5-58273), shows significant staining of MAP4 in human skeletal muscle and shows minimal or weak staining in human liver tissues. The relative expression levels of MAP4 within each tissue is shown using RNA-Seq. {RE}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

0.25-2 µg/mL

Immunofluorescence (IF)

0.25-2 µg/mL

Immunohistochemistry (IHC)

1:2500-1:5000

Product Specifications

Species

Human

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human MAP4

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2643742

Product Specific Information

Immunogen sequence: PPAQALEIMM GLKTTDMAPS KETEMALAKD MALATKTEVA LAKDMESPTK LDVTLAKDMQ PSMESDMALV KDMELPTEKE VALVKDVRWP TETDVSSAKN VV

Highest antigen sequence identity to the following orthologs: Mouse - 48%, Rat - 47%.

Target Information

The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.