Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
MAPKAP1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 1
MAPKAP1 Antibody (PA1-41148) in WB
Western blot analysis of MAPKAP1 in human heart lysate in the 1) absence and 2) presence of immunizing peptide and in human skeletal muscle lysate in the 3) absence and 4) presence of immunizing peptide. Samples were incubated in MAPKAP1 polyclonal antibody (Product # PA1-41148) using a dilution of 5 µg/mL.
Product Details
PA1-41148
Product Specifications
Species Reactivity
Avian,
Bovine,
Dog,
Chicken,
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A portion of amino acids 85-102 of human Mip1.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein G
Storage buffer
PBS with 0.2% gelatin
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: human skeletal muscle and heart protein or cell lysate.
Immunogen sequence: NTAQRLERLR KERQNQIK
Target Information
Sin1 was initially identified as the human homolog of S. pombe SIN1. Recent evidence has shown that it identical to Mip1, a protein that interacts with MEKK2, a member of the mitogen-activated protein kinase (MAPK) intracellular signaling network. MAPKAP1 is thought to prevent MEKK2 activation and dimerization by forming a complex with the inactive and non-phosphorylated MEKK2, thereby blocking the JNK1/2, ERK1/2, p38 and ERK5 MAPKs. MAPKAP1 has also been shown to play a role in the TOR signaling process, a pathway that is involved in controlling cell growth and proliferation in response to environmental cues such as nutrients, growth factors and hormones. Experiments showed that MAPKAP1 helped to maintain the TOR/rictor assembly but not the TOR/RAPTOR complex, which allowed specific phosphorylation of Akt, a kinase that is believed to couple the growth factor-PI3K signaling pathway to the nutrient-regulated TOR signaling pathway. Multiple alternatively spliced isoforms of MAPKAP1 have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: MEKK2-interacting protein 1; MGC2745; MIP1SIN1SIN1b; mitogen activated protein kinase associated protein 1; Mitogen-activated protein kinase 2-associated protein 1; mitogen-activated protein kinase associated protein 1; mSIN1; ras inhibitor MGC2745; RP11-269P11.1; SAPK interacting protein; SAPK-interacting protein 1; stress-activated map kinase interacting protein 1; Stress-activated map kinase-interacting protein 1; stress-activated protein kinase-interacting 1; Target of rapamycin complex 2 subunit MAPKAP1; TORC2 subunit MAPKAP1
Gene Aliases: AI591529; D230039K05Rik; im:7143088; JC310; MAPKAP1; MIP1; si:ch73-242f23.1; SIN1; SIN1b; SIN1g
UniProt ID: (Chicken) Q9W6S3, (Bovine) A2VDU2, (Human) Q9BPZ7, (Rat) Q6AYF1, (Mouse) Q8BKH7
Entrez Gene ID: (Chicken) 395627, (Dog) 480724, (Bovine) 533861, (Human) 79109, (Rat) 296648, (Mouse) 227743, (Zebrafish) 100537196
phosphatidylinositol-4,5-bisphosphate binding
phosphatidylinositol-3,4,5-trisphosphate binding
Ras GTPase binding
protein kinase binding
phosphatidylinositol-3,4-bisphosphate binding
phosphatidic acid binding
phosphatidylinositol-3,5-bisphosphate binding
protein binding
molecular_function
scaffold/adaptor protein
establishment or maintenance of actin cytoskeleton polarity
positive regulation of peptidyl-serine phosphorylation
TORC2 signaling
negative regulation of Ras protein signal transduction
regulation of cellular response to oxidative stress
substantia nigra development
T cell costimulation
phosphatidylinositol-mediated signaling
biological_process
regulation of cell projection assembly
cell migration involved in mesendoderm migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support