Western blot analysis of MAPKAPK2 in extracts of rat skeletal muscle. Samples were incubated with MAPKAPK2 Polyclonal antibody (Product # PA5-109937) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

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Immunogen image

Immunogen Image MAPKAPK2 Polyclonal Antibody

Product Details

PA5-109937

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

ELISA (ELISA)

1 µg/mL

Product Specifications

Species Reactivity

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human MAPKAPK-2/MK2 (NP_1165842)

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.88 mg/mL

188 µg

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2855348

Product Specific Information

Immunogen sequence: MLIRNLLKTE PTQRMTITEF MNHPWIMQST KVPQTPLHTS RVLKEDKERW EDVKEEMTSA LATMRVDYEQ IKIKKIEDAS NPLLLKRRKK ARALEAAALA H

Target Information

MAPKAPK2 mediates p38 and ERK signaling in vivo. It is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation, and cell proliferation. MAPKAPK2 encodes a member of the Ser/Thr protein kinase family. MAPKAPK2 is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, MAPKAPK2 is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for MAPKAPK2.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MAP kinase-activated protein kinase 2

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Entrez Gene ID: (Rat) 289014

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Function(s)

protein serine/threonine kinase activity ATP binding non-receptor serine/threonine protein kinase

Process(es)

toll-like receptor signaling pathway protein phosphorylation inflammatory response cellular response to DNA damage stimulus peptidyl-serine phosphorylation response to lipopolysaccharide regulation of interleukin-6 production regulation of tumor necrosis factor production response to cytokine cellular response to vascular endothelial growth factor stimulus macropinocytosis vascular endothelial growth factor receptor signaling pathway mRNA stabilization inner ear development 3'-UTR-mediated mRNA stabilization

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MAPKAPK2 Polyclonal Antibody

MAPKAPK2 Antibody (PA5-109937) in WB