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Product Details
PA5-23146
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Horse,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A portion of amino acids 300-350 of human RNF174
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 0.2% gelatin
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2540673
Target Information
RNF174 (MARCH4) is a multi-pass membrane protein mainly found in the membrane of Golgi apparatus. It is a complex enzyme with a RING-CH-type zinc finger. It acts as a ubiquitin-protein ligase mainly involved in third step of ubiquitin conjugation. The RINGv-type zinc finger domain is required for E3 ligase activity in which the E3 ubiquitin-protein ligase mediates ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. It is mainly expressed in brain and placenta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase MARCH4; E3 ubiquitin-protein ligase MARCHF4; MARCH-IV; membrane associated ring finger 4; membrane-associated ring finger (C3HC4) 4, E3 ubiquitin protein ligase; Membrane-associated RING finger protein 4; Membrane-associated RING-CH protein IV; RING finger protein 174; RING-type E3 ubiquitin transferase MARCHF4
Gene Aliases: BC056494; KIAA1399; MARCH-IV; MARCH4; MARCHF4; mKIAA1399; RNF174
UniProt ID: (Human) Q9P2E8, (Mouse) Q80TE3
Entrez Gene ID: (Human) 57574, (Horse) 100050538, (Rat) 367295, (Mouse) 381270
ubiquitin-protein transferase activity
zinc ion binding
ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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