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Invitrogen
MARCH8 Polyclonal Antibody
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Product Details
PA5-20632
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 17 amino acid peptide from near the carboxy terminus of human MARCH8.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is Hela cell lysate.
PA5-20632 can be used with blocking peptide PEP-0752.
Target Information
MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: c-MIR; c-mir, cellular modulator of immune recognition; Cellular modulator of immune recognition; cellular modulator of immune recognition (c-MIR); E3 ubiquitin-protein ligase MARCH8; E3 ubiquitin-protein ligase MARCHF8; MARCH-VIII; membrane associated ring finger 8; membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase; Membrane-associated RING finger protein 8; Membrane-associated RING-CH protein VIII; RING finger protein 178; RING-type E3 ubiquitin transferase MARCHF8; RP11-67C2.1
Gene Aliases: 1300017E09Rik; c-MIR; CMIR; MARCH-VIII; MARCH8; MARCHF8; MIR; RNF178
UniProt ID: (Human) Q5T0T0, (Mouse) Q9DBD2
Entrez Gene ID: (Human) 220972, (Mouse) 71779, (Rat) 312656
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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