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MARCH9 Antibody (PA5-88012) in WB
Western blot analysis of MARCH9 in 293T cells. Samples were incubated with MARCH9 Polyclonal antibody (Product # PA5-88012) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88012
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 277-346 of human MARCH9 (NP_6124052)
Conjugate
Form
Liquid
Concentration
2.04 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804586
Product Specific Information
Immunogen sequence: GTAGKSGPRN SRTGPTSGAT SRPPAAQRMR TLLPQRCGYT ILHLLGQLRP PDARSSSHSG REVVMRVTTV; Positive Samples: U-251MG, A-549, 293T, Mouse intestine, Mouse kidney, Mouse eye, Rat kidney, Rat eye; Cellular Location: Golgi apparatus membrane, Lysosome membrane, Multi-pass membrane protein
Target Information
MARCH9 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH9 induces internalization of several membrane glycoproteins and directs them to the endosomal compartment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 9-Mar; E3 ubiquitin-protein ligase MARCHF9; MARCH-IX; membrane associated ring finger 9; membrane-associated ring finger (C3HC4) 9; Membrane-associated RING finger protein 9; Membrane-associated RING-CH protein IX; RING finger protein 179; RING-type E3 ubiquitin transferase MARCHF9
Gene Aliases: MARCH-IX; MARCH9; MARCHF9; RNF179
UniProt ID: (Human) Q86YJ5
Entrez Gene ID: (Human) 92979
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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