Immunofluorescence staining of mixed neuron/glial cultures with anti-MARCKS antibody (PA1-10021) (Red) and anti-MAP2 antibody (PA1-10005) (green). Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.
|Tested species reactivity||Bovine, Chicken, Horse, Human, Mouse, Pig, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full-length recombinant human MARCKS|
|Storage buffer||whole serum|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Prion Protein Deficiency Causes Diverse Proteome Shifts in Cell Models That Escape Detection in Brain Tissue.
PA1-10021 was used in western blot to study the cause of diverse proteome shifts in cell models that escape detection in brain tissue with prion protein deficiency
|Mehrabian M,Brethour D,Williams D,Wang H,Arnould H,Schneider B,Schmitt-Ulms G||PloS one (11:null)||2016|